鲨鱼粉尘:加工残留物高通量DNA测序在受威胁鲨鱼和鳐鱼贸易监测中的应用

IF 7.7 1区 环境科学与生态学 Q1 BIODIVERSITY CONSERVATION Conservation Letters Pub Date : 2023-07-19 DOI:10.1111/conl.12971
Andhika P. Prasetyo, Joanna M. Murray, Muh. Firdaus A. K. Kurniawan, Naiara G. Sales, Allan D. McDevitt, Stefano Mariani
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引用次数: 0

摘要

非法捕鱼、不受监管的副渔获物和市场对某些产品(如鱼鳍)的需求是全球鲨鱼和鳐鱼数量迅速下降的主要原因。由于产品的多样性、分类的模糊性和贸易的复杂性,控制濒危物种的贸易仍然很困难。传统上用于识别交易物种的遗传工具通常以个体组织样本为目标,并且耗时和/或具有物种特异性。在这里,我们对从贸易活动留下的灰尘和废料中提取的微量DNA片段进行了高通量测序。我们对世界上最大的鲨鱼登陆地(印度尼西亚爪哇岛)的七家加工厂的“鲨鱼尘”样本进行了MetaBarcode编码,并确定了61个鲨鱼和鳐鱼分类群(代表所有软骨鱼目的一半),其中一半以上无法从同一地点同时采集的组织样本中回收。重要的是,超过80%的鲨鱼尘埃序列被发现属于《濒危野生动植物种公约》所列物种。我们认为,无论野生动物在哪里交易,这种方法都可能成为一种强大且具有成本效益的监测工具。
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Shark-dust: Application of high-throughput DNA sequencing of processing residues for trade monitoring of threatened sharks and rays

Illegal fishing, unregulated bycatch, and market demand for certain products (e.g., fins) are largely responsible for the rapid global decline of shark and ray populations. Controlling trade of endangered species remains difficult due to product variety, taxonomic ambiguity, and trade complexity. The genetic tools traditionally used to identify traded species typically target individual tissue samples, and are time-consuming and/or species-specific. Here, we performed high-throughput sequencing of trace DNA fragments retrieved from dust and scraps left behind by trade activities. We metabarcoded “shark-dust” samples from seven processing plants in the world's biggest shark landing site (Java, Indonesia), and identified 61 shark and ray taxa (representing half of all chondrichthyan orders), more than half of which could not be recovered from tissue samples collected in parallel from the same sites. Importantly, over 80% of shark-dust sequences were found to belong to CITES-listed species. We argue that this approach is likely to become a powerful and cost-effective monitoring tool wherever wildlife is traded.

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来源期刊
Conservation Letters
Conservation Letters BIODIVERSITY CONSERVATION-
CiteScore
13.50
自引率
2.40%
发文量
70
审稿时长
>12 weeks
期刊介绍: Conservation Letters is a reputable scientific journal that is devoted to the publication of both empirical and theoretical research that has important implications for the conservation of biological diversity. The journal warmly invites submissions from various disciplines within the biological and social sciences, with a particular interest in interdisciplinary work. The primary aim is to advance both pragmatic conservation objectives and scientific knowledge. Manuscripts are subject to a rapid communication schedule, therefore they should address current and relevant topics. Research articles should effectively communicate the significance of their findings in relation to conservation policy and practice.
期刊最新文献
Issue Information Rapid population response to a hunting ban in a previously overharvested, threatened landbird Lead-based ammunition is a threat to the endangered New Zealand Kea (Nestor notabilis) Reframing conservation audiences from individuals to social beings Identifying climate-smart tropical Key Biodiversity Areas for protection in response to widespread temperature novelty
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