视网膜退化的啮齿类动物模型:从培养的纯化细胞到活体动物。

IF 7.8 2区 医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL Cold Spring Harbor perspectives in medicine Pub Date : 2024-10-01 DOI:10.1101/cshperspect.a041311
Valérie Fradot, Sébastien Augustin, Valérie Fontaine, Katia Marazova, Xavier Guillonneau, José A Sahel, Serge Picaud
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引用次数: 0

摘要

视网膜变性的啮齿动物模型对于制定治疗策略至关重要。除了活体动物模型外,我们在这里还讨论了基于啮齿动物细胞培养的模型,如纯化的视网膜神经节细胞和视网膜外植体。这些离体模型通过消除药物药代动力学和生物利用度方面的问题,扩展了研究病理机制和评估药理学药物的神经保护作用的可能性。活体啮齿动物模型的数量大大增加,有可能在动物身上实现转基因修饰,以敲除基因和突变。Cre-lox系统进一步使研究人员能够在特定阶段靶向特定细胞中的特定基因或突变。然而,化学或物理诱导的模型可以提供这种靶向基因修饰的替代方案。啮齿动物模型的多样性增加了我们解决大多数眼部病理的可能性,为创新治疗策略的概念提供了初步的证明。
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Rodent Models of Retinal Degeneration: From Purified Cells in Culture to Living Animals.

Rodent models of retinal degeneration are essential for the development of therapeutic strategies. In addition to living animal models, we here also discuss models based on rodent cell cultures, such as purified retinal ganglion cells and retinal explants. These ex vivo models extend the possibilities for investigating pathological mechanisms and assessing the neuroprotective effect of pharmacological agents by eliminating questions on drug pharmacokinetics and bioavailability. The number of living rodent models has greatly increased with the possibilities to achieve transgenic modifications in animals for knocking in and out genes and mutations. The Cre-lox system has further enabled investigators to target specific genes or mutations in specific cells at specific stages. However, chemically or physically induced models can provide alternatives to such targeted gene modifications. The increased diversity of rodent models has widened our possibility to address most ocular pathologies for providing initial proof of concept of innovative therapeutic strategies.

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来源期刊
Cold Spring Harbor perspectives in medicine
Cold Spring Harbor perspectives in medicine MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
14.30
自引率
1.90%
发文量
44
审稿时长
4-8 weeks
期刊介绍: Cold Spring Harbor Perspectives in Medicine is a monthly online publication comprising reviews on different aspects of a variety of diseases, covering everything from the molecular and cellular bases of disease to translational medicine and new therapeutic strategies. Cold Spring Harbor Perspectives in Medicine is thus unmatched in its depth of coverage and represents an essential source where readers can find informed surveys and critical discussion of advances in molecular medicine.
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