对成年小鼠热应激精子自噬的抑制:Catsper1,2通道蛋白的可能作用。

IF 2.1 4区 医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Journal of Tropical Medicine Pub Date : 2023-10-09 eCollection Date: 2023-01-01 DOI:10.1155/2023/6890815
Malihe Soltani, Majid Rahmati, Mohammad Reza Nikravesh, Shahin Saeedi Nejat, Mahdi Jalali
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引用次数: 0

摘要

目的:各种现象保证了配子在进化的各个阶段的成熟和形成,其中之一是自噬,它在配子,特别是精子的最终形态中发挥着关键作用。自噬受到氧化应激、钙稳态紊乱和高温条件的影响。目前的研究旨在评估热应激(HS)诱导后的自噬相关蛋白以及精子钙通道(CatSper)蛋白的活性。方法:研究样本包括两组成年小鼠:假手术组和HS组。在HS组中,将右侧睾丸转移到腹腔120 小时,然后回到阴囊,在那里停留了7个小时 天。7点之后 第天,取睾丸和附睾进行实时、免疫组织化学研究、精子参数评估和生精小管评估。在本研究中,测量了自噬蛋白的表达和分布。此外,CatSper1和CatSper2被评估为钙通道的蛋白质。结果:本研究结果表明,HS诱导后,曲精管中自噬指数的表达强度显著降低,这与CatSper蛋白在精子中的分布减少有关。HS导致精子的形态学变化,降低精子的活力和活力,降低精子发生指数。结论:在本研究中,热应激后CatSper蛋白分布的减少可能导致CatSper通道的结构紊乱,从而强烈影响自噬活性。此外,精子发生和精子参数的破坏可能是自噬活性降低的结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Inhibition of Autophagy in Heat-Stressed Sperm of Adult Mice: A Possible Role of Catsper1, 2 Channel Proteins.

Objective: Various phenomena guarantee gamete maturation and formation at all stages of evolution, one of which is autophagy playing a critical role in the final morphology of gametes, particularly sperms. Autophagy is influenced by oxidative stress, disturbances of calcium homeostasis, and hyperthermia conditions. The current study aimed to assess the autophagy-related proteins along with the activity of sperm calcium channel (CatSper) proteins following the induction of heat stress (HS).

Methods: The study sample includes two groups of adult mice: sham and HS groups. In the HS group, the right testis was transferred to the abdominal cavity for 120 hours and then returned to the scrotum where it remained for 7 days. After 7 days, the testis and epididymis were removed to conduct real-time, immunohistochemical studies, sperm parameter evaluation, and seminiferous tubule assessment. In this study, the expression and distribution of autophagy proteins were measured. Plus, CatSper1 and CatSper2 were evaluated as proteins of calcium channels.

Results: The results of the present study demonstrated that the expression intensity of autophagy indices in seminiferous tubules decreased significantly after HS induction, which was associated with a decrease in the distribution of CatSper proteins in the sperms. HS led to morphological changes in sperm, reduced motility and viability of sperm, and decreased spermatogenesis indices.

Conclusion: In this study, following heat stress, the decrease in CatSper protein distribution may lead to the structural disorder of CatSper channels, which could strongly affect autophagic activity. Also, disruption of spermatogenesis and sperm parameters may be the consequence of decreased autophagy activity.

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来源期刊
Journal of Tropical Medicine
Journal of Tropical Medicine Immunology and Microbiology-Parasitology
CiteScore
3.90
自引率
4.50%
发文量
0
审稿时长
14 weeks
期刊介绍: Journal of Tropical Medicine is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on all aspects of tropical diseases. Articles on the pathology, diagnosis, and treatment of tropical diseases, parasites and their hosts, epidemiology, and public health issues will be considered. Journal of Tropical Medicine aims to facilitate the communication of advances addressing global health and mortality relating to tropical diseases.
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