E. T. Setiatin, D. A. Lestari, D. W. Harjanti, A. Setiaji, E. Kurnianto
{"title":"促卵泡激素受体基因探索作为印尼当地山羊高产性状的可能标记","authors":"E. T. Setiatin, D. A. Lestari, D. W. Harjanti, A. Setiaji, E. Kurnianto","doi":"10.14710/jitaa.48.1.28-36","DOIUrl":null,"url":null,"abstract":"The objective of this study was to discover and identify the effect of SNP of follicle-stimulating hormone receptor (FSHR) gen on prolific traits in Kejobong (KJ) and Etawah grade (EG) doe. A total of 15 blood samples were taken from 11 KJ and 4 EG with various parity and type of birth. The FSHR gene was amplified from the DNA template by Polymerase Chain Reaction (PCR); the PCR products were then sequenced to determine Single Nucleotide Polymorphisms (SNP). Results showed that 3 SNPs were identified, and those SNPs altered amino acid sequence formed 6 haplotypes and divided the doe based on the type of birth. In conclusion, SNPs identified in this study are associated with a prolific trait that can be used as a genetic marker and haplotype 3, 4, and 5 showed the highest prolific on KJ and EG doe.","PeriodicalId":17297,"journal":{"name":"Journal of the Indonesian Tropical Animal Agriculture","volume":"7 13","pages":""},"PeriodicalIF":0.3000,"publicationDate":"2023-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Follicle-stimulating hormone receptor gene exploration as possible markers for prolific trait of local goat in Indonesia\",\"authors\":\"E. T. Setiatin, D. A. Lestari, D. W. Harjanti, A. Setiaji, E. Kurnianto\",\"doi\":\"10.14710/jitaa.48.1.28-36\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The objective of this study was to discover and identify the effect of SNP of follicle-stimulating hormone receptor (FSHR) gen on prolific traits in Kejobong (KJ) and Etawah grade (EG) doe. A total of 15 blood samples were taken from 11 KJ and 4 EG with various parity and type of birth. The FSHR gene was amplified from the DNA template by Polymerase Chain Reaction (PCR); the PCR products were then sequenced to determine Single Nucleotide Polymorphisms (SNP). Results showed that 3 SNPs were identified, and those SNPs altered amino acid sequence formed 6 haplotypes and divided the doe based on the type of birth. In conclusion, SNPs identified in this study are associated with a prolific trait that can be used as a genetic marker and haplotype 3, 4, and 5 showed the highest prolific on KJ and EG doe.\",\"PeriodicalId\":17297,\"journal\":{\"name\":\"Journal of the Indonesian Tropical Animal Agriculture\",\"volume\":\"7 13\",\"pages\":\"\"},\"PeriodicalIF\":0.3000,\"publicationDate\":\"2023-02-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of the Indonesian Tropical Animal Agriculture\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.14710/jitaa.48.1.28-36\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Indonesian Tropical Animal Agriculture","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.14710/jitaa.48.1.28-36","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Follicle-stimulating hormone receptor gene exploration as possible markers for prolific trait of local goat in Indonesia
The objective of this study was to discover and identify the effect of SNP of follicle-stimulating hormone receptor (FSHR) gen on prolific traits in Kejobong (KJ) and Etawah grade (EG) doe. A total of 15 blood samples were taken from 11 KJ and 4 EG with various parity and type of birth. The FSHR gene was amplified from the DNA template by Polymerase Chain Reaction (PCR); the PCR products were then sequenced to determine Single Nucleotide Polymorphisms (SNP). Results showed that 3 SNPs were identified, and those SNPs altered amino acid sequence formed 6 haplotypes and divided the doe based on the type of birth. In conclusion, SNPs identified in this study are associated with a prolific trait that can be used as a genetic marker and haplotype 3, 4, and 5 showed the highest prolific on KJ and EG doe.
期刊介绍：
Journal of the Indonesian Tropical Animal Agriculture (JITAA) is a double blind peer-reviewed publication devoted to disseminate all information contributing to the understanding and development of animal agriculture in the tropics by publication of original research papers. The journal covers all aspect relating to Animal Agriculture, including: -Animal breeding and genetics -Animal feeding and nutrition -Animal reproduction -Animal biotechnology -Animal physiology -Animal production and technology -Animal products technology -Animal management and economics -Animal products processing and animal by-products -Animal microbiology -Livestock farming systems -Other related topics in relation to animal science. The topics of research are not only on Indonesian tropical animal agriculture, but also on other tropical regions of the world.
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