广泛耐药鲍曼不动杆菌生物膜相关基因的表达

IF 0.5 4区 医学 Q4 MICROBIOLOGY Jundishapur Journal of Microbiology Pub Date : 2023-06-13 DOI:10.5812/jjm-133999
Maryam Khosravy, F. Hosseini, M. Razavi, Ramazan Ali Khavari
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引用次数: 0

摘要

背景:鲍曼不动杆菌是长期机械通气患者院内肺炎的重要病因。此外,广泛耐药(XDR)菌株引起重症监护病房(ICU)患者感染。鲍曼不动杆菌的慢性感染和抗菌素耐药性与生物膜的形成有关。几种毒力基因,如blaPER-1、pgaA和bap,都参与了生物膜的形成。目的:本研究检测肺炎患者生物膜形成相关基因的表达水平。方法:收集ICU住院患者的痰液,分离鲍曼不动杆菌ATCC 19606作为参考菌株,分别在血琼脂、伊红亚甲基蓝琼脂和巧克力琼脂培养基上培养。培养基在37℃下孵育18 - 24小时。接下来,从伊朗德黑兰Besat医院ICU患者的痰样本中收集革兰氏30株XDR鲍曼尼杆菌。对分离的细菌进行了抗生素抗性模式和生物膜形成能力的表征。随后,从形成生物膜的分离株中提取RNA。实时聚合酶链反应(PCR)检测blaPER-1、pgaA和bap基因的表达水平。转录本归一化为16S rRNA作为内控,计算基因表达折叠变化。统计学分析采用SPSS (V. 16)非配对双尾t检验(P < 0.05)。结果:膜片扩散药敏试验显示,30株(100%)分离菌对哌拉西林-他唑巴坦、头孢吡肟、头孢曲松、头孢他啶、庆大霉素、亚胺培南、美罗培南、左氧氟沙星、环丙沙星均耐药。所有来自icu住院患者的30株分离株(100%)被归类为XDR, 27株(90%)分离株显示能够形成生物膜。得到的结果表明基因表达水平有显著差异。blaPER-1、bap和pgaA的表达倍数变化分别为7.473、11.964和5.277。结论:在我们的研究中,XDR鲍曼杆菌主要引起呼吸机相关性肺炎,并且在这些菌株中观察到生物膜相关基因的表达增加。医疗保健中心应实施适当的感染控制方案,以管理院内感染,特别是在ICU。
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Expression of Biofilm-Related Genes in Extensively Drug-Resistant Acinetobacter baumannii
Background: Acinetobacter baumannii is an important cause of nosocomial pneumonia in patients requiring long-term mechanical ventilation. Besides, extensively drug-resistant (XDR) strains cause infection in intensive care unit (ICU) patients. Chronic infections of A. baumannii and antimicrobial resistance are associated with biofilm formation. Several virulence genes, such as blaPER-1, pgaA, and bap, are involved in biofilm formation. Objectives: The current study examines the expression levels of biofilm formation-related genes in pneumonia patients. Methods: The sputum samples were collected from patients hospitalized in the ICU, and A. baumannii ATCC 19606, the reference strain, was isolated and cultured on blood agar, eosin methylene blue agar, and chocolate agar medium. The media were then incubated at 37°C for 18 - 24 hours. Next, Gram-Thirty XDR A. baumannii isolates were collected from the sputum samples of ICU patients at Besat Hospital in Tehran, Iran. Bacterial isolates were characterized for antibiotic resistance patterns and biofilm-forming ability. Subsequently, RNA was extracted from the biofilm-forming isolates. A real-time polymerase chain reaction (PCR) assay was performed to evaluate the expression levels of the blaPER-1, pgaA, and bap genes. Transcripts were normalized to 16S rRNA as an internal control, and gene expression fold changes were calculated. Statistical analysis was performed using an unpaired two-tailed t-test (P < 0.05) with SPSS (V. 16). Results: The disk diffusion susceptibility test revealed that all 30 (100%) isolates were resistant to piperacillin-tazobactam, cefepime, ceftriaxone, ceftazidime, gentamicin, imipenem, meropenem, levofloxacin, and ciprofloxacin. All 30 isolates from ICU-admitted patients (100%) were classified as XDR, and 27 (90%) isolates demonstrated the ability to form biofilms. The obtained results indicated a significant difference in gene expression levels. The fold change in expression for blaPER-1, bap, and pgaA was 7.473, 11.964, and 5.277, respectively. Conclusions: In our study, XDR A. baumannii primarily caused ventilator-associated pneumonia, and an observed increase in the expression of biofilm-related genes was noted in these strains. Healthcare centers should implement appropriate infection control programs to manage nosocomial infections, particularly in the ICU.
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来源期刊
CiteScore
1.30
自引率
0.00%
发文量
56
审稿时长
6-12 weeks
期刊介绍: Jundishapur Journal of Microbiology, (JJM) is the official scientific Monthly publication of Ahvaz Jundishapur University of Medical Sciences. JJM is dedicated to the publication of manuscripts on topics concerning all aspects of microbiology. The topics include medical, veterinary and environmental microbiology, molecular investigations and infectious diseases. Aspects of immunology and epidemiology of infectious diseases are also considered.
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