长期干旱对毛果杨耐旱和耐旱基因型地下微生物组的影响

IF 3.3 3区 生物学 Q2 MICROBIOLOGY Phytobiomes Journal Pub Date : 2022-07-13 DOI:10.1094/pbiomes-12-21-0076-r
Brandon David Kristy, A. Carrell, E. Johnston, D. Klingeman, K. Gwinn, Kimberly C. Syring, Caroline Skalla, Scott Emrich, M. Cregger
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引用次数: 4

摘要

毛果杨是一种重要的生态树种和重要的经济生物饲料。在地下,毛果P.trichocarpa与根际和根内的各种微生物相互作用。这些植物与微生物的相互作用可以促进植物的各种过程,从抑制病原体到耐旱,但我们对长期干旱胁迫对毛果霉地下微生物群的影响知之甚少。为了研究长期干旱对遗传不同的毛果P.trichocarpa宿主地下微生物群落的相互作用,我们在美国俄勒冈州Boardman的一项长期干旱实验中评估了所选基因型的根内、根际和周围大块土壤中的古菌/细菌和真菌群落。我们对从16种不同的毛果P.trichocarpa基因型采集的样本上的16S rRNA和ITS2基因区进行了测序,这些样本位于灌溉充足或减少灌溉的地块中。其中8种基因型先前已被鉴定为耐旱基因型,而其他8种基因则易感干旱。虽然减少灌溉会影响每个古菌/细菌微生物组隔间的组成,但真菌群落仅在根际和大块土壤隔间受到影响。耐旱细菌,如放线菌,在所有地下微生物群中,在减少灌溉的情况下含量不同。寄主耐旱性影响植物相关微生物组区室,但对大块土壤区室影响不大。耐旱树木在根内和根际富集了潜在的促生长微生物,包括鞘氨醇单胞菌和外生菌根真菌。总的来说,在易发生周期性干旱的地区,可以利用耐旱毛果P.trichocarpa基因型中促生长微生物的关联来提高生物饲料生产力。
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Chronic drought differentially alters the belowground microbiome of drought tolerant and drought susceptible genotypes of Populus trichocarpa
Populus trichocarpa is an ecologically important tree species and economically important biofeedstock. Belowground, P. trichocarpa interacts with diverse microorganisms in the rhizosphere and root endosphere. These plant-microbial interactions can bolster a variety of plant processes, ranging from pathogen suppression to drought tolerance, yet we know little about the impact of chronic drought stress on P. trichocarpa’s belowground microbiomes. To investigate the interactive effect of chronic drought on belowground microbial communities across genetically different P. trichocarpa hosts, we assessed archaeal/bacterial and fungal communities within the root endosphere, rhizosphere, and surrounding bulk soil of selected genotypes in a long-term drought experiment in Boardman, OR, USA. We sequenced the 16S rRNA and ITS2 gene region on samples collected from 16 distinct P. trichocarpa genotypes in plots with full or reduced irrigation. Eight of these genotypes have been previously identified as drought tolerant while the other eight genotypes were drought susceptible. While reduced irrigation influenced the composition of every archaeal/bacterial microbiome compartment, fungal communities were only affected in the rhizosphere and bulk soil compartments. Drought-tolerant bacteria, such as Actinobacteria, were differentially abundant in reduced irrigation across all belowground microbiomes. Host drought-tolerance influenced plant-associated microbiome compartments but had little impact on the bulk soil compartment. Drought-tolerant trees were enriched for potential growth-promoting microorganisms in the root endosphere and rhizosphere, including Sphingomonas bacteria and ectomycorrhizal fungi. Overall, associations of growth-promoting microbes in drought resistant P. trichocarpa genotypes can be leveraged to improve biofeedstock productivity in regions prone to periodic drought.
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来源期刊
CiteScore
7.40
自引率
6.80%
发文量
42
审稿时长
4 weeks
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