{"title":"卡帕-卡拉胶交联磁性叶酸共轭壳聚糖纳米复合材料用于精氨酸酶包封、递送和癌症治疗","authors":"Rahman Moradi, R. Mohammadzadeh, A. Akbari","doi":"10.1142/s1793984421400055","DOIUrl":null,"url":null,"abstract":"In this study, the Aspergillus niger L-arginase was expressed in Pichia pastorise and immobilized on Kappa-carrageenan crosslinked magnetic folic acid-conjugated chitosan nanoparticles (magnetic-FA/CTS NPs). L-arginase gene was isolated and cloned in pGAPZ[Formula: see text]A plasmid and transformed in P. pastoris. The purified L-arginase was loaded on magnetic-FA/CTS NPs nanocomposites and the structure of the synthesized nano-carriers was investigated using SEM, FT-IR and DLS techniques. Prepared formulations showed high encapsulation efficiency (91.93%). In vitro release and cytotoxic studies were performed at different pH (pH of 4–8) and against Molt-4 cancer cell line, respectively.","PeriodicalId":44929,"journal":{"name":"Nano Life","volume":"1 1","pages":""},"PeriodicalIF":0.8000,"publicationDate":"2021-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"Kappa-Carrageenan Crosslinked Magnetic Folic Acid-Conjugated Chitosan Nanocomposites for Arginase Encapsulation, Delivery and Cancer Therapy\",\"authors\":\"Rahman Moradi, R. Mohammadzadeh, A. Akbari\",\"doi\":\"10.1142/s1793984421400055\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In this study, the Aspergillus niger L-arginase was expressed in Pichia pastorise and immobilized on Kappa-carrageenan crosslinked magnetic folic acid-conjugated chitosan nanoparticles (magnetic-FA/CTS NPs). L-arginase gene was isolated and cloned in pGAPZ[Formula: see text]A plasmid and transformed in P. pastoris. The purified L-arginase was loaded on magnetic-FA/CTS NPs nanocomposites and the structure of the synthesized nano-carriers was investigated using SEM, FT-IR and DLS techniques. Prepared formulations showed high encapsulation efficiency (91.93%). In vitro release and cytotoxic studies were performed at different pH (pH of 4–8) and against Molt-4 cancer cell line, respectively.\",\"PeriodicalId\":44929,\"journal\":{\"name\":\"Nano Life\",\"volume\":\"1 1\",\"pages\":\"\"},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2021-06-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nano Life\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1142/s1793984421400055\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MATERIALS SCIENCE, BIOMATERIALS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nano Life","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1142/s1793984421400055","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
Kappa-Carrageenan Crosslinked Magnetic Folic Acid-Conjugated Chitosan Nanocomposites for Arginase Encapsulation, Delivery and Cancer Therapy
In this study, the Aspergillus niger L-arginase was expressed in Pichia pastorise and immobilized on Kappa-carrageenan crosslinked magnetic folic acid-conjugated chitosan nanoparticles (magnetic-FA/CTS NPs). L-arginase gene was isolated and cloned in pGAPZ[Formula: see text]A plasmid and transformed in P. pastoris. The purified L-arginase was loaded on magnetic-FA/CTS NPs nanocomposites and the structure of the synthesized nano-carriers was investigated using SEM, FT-IR and DLS techniques. Prepared formulations showed high encapsulation efficiency (91.93%). In vitro release and cytotoxic studies were performed at different pH (pH of 4–8) and against Molt-4 cancer cell line, respectively.
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