Yufen Tao, Xue Han, Nannan Liu, Lei Shi, Li Shi, Shuyuan Liu, Yufeng Yao
{"title":"TAP和hla - 1基因组合与中国汉族慢性丙型肝炎病毒感染的相关性研究","authors":"Yufen Tao, Xue Han, Nannan Liu, Lei Shi, Li Shi, Shuyuan Liu, Yufeng Yao","doi":"10.1111/iji.12574","DOIUrl":null,"url":null,"abstract":"<p>Host immune system genes play key roles in the progression of chronic hepatitis C virus (HCV) infection. Transporters associated with antigen processing (TAP) play an important role in the loading of viral peptides onto MHC class I molecules. This study aimed to investigate the association between <i>TAP</i> gene polymorphisms and chronic HCV in a Chinese Han population. A total of 232 chronic hepatitis C (CHC) patients and 362 healthy individuals were recruited from the Han population in Yunnan province in southwest China, and a TaqMan SNP genotyping assay was used to detect six single nucleotide polymorphisms (SNPs) of <i>TAP1</i> and three SNPs of <i>TAP2</i> genes. The association of the <i>TAP</i> gene with CHC was analysed at the allele, genotype, and haplotype levels. There were no significant differences in the allele and genotype frequencies of these SNPs in the <i>TAP</i> gene between CHC patients and controls after Bonferroni correction. A novel <i>TAP1</i> allele (<i>TAP1*unknown_1</i>: rs41555220-rs41549617-rs1057141-rs1135216-rs1057149-rs41551515: G-G-A-G-G-G) was only present in the CHC group, and this allele significantly increased susceptibility to CHC (<i>p </i>= .005, odds ratio [OR] = 11.105. 95% confidence interval [CI]: 1.362–90.558). Homozygous <i>TAP1*03:01/TAP1*03:01</i> was observed only in the CHC group that exhibited an obvious risk for CHC (<i>p </i>= .002, OR = 9.637, 95% CI: 1.153–80.574). And the haplotype <i>TAP1*unknown_1-TAP2*01:01</i> was only present in the CHC group and indicated a significant risk for CHC (<i>p </i>= .002, OR = 9.498, 95% CI: 1.140–79.149). We observed significant interactions among <i>HLA-A</i>, <i>-B</i>,<i>C</i>, <i>TAP1</i>, and <i>TAP2</i> alleles, and combination analysis revealed that the combination of <i>TAP1*01:01-TAP2*01:01-HLA-B*35:01</i> was only present in the control group (2.2%) and resulted in significantly increased resistance to CHC (<i>p </i>= .002, OR = 0.096, 95% CI: 0.012–0.759). Whereas, the combination of <i>TAP1*01:01-TAP2*01:01-HLA-C*07:02</i> and <i>TAP1*03:01-TAP2*01:01-HLA-C*01:02</i> increased the susceptibility to CHC significantly (<i>p </i>= .001, OR = 2.016, 95% CI: 1.309–3.106 and <i>p </i>= .002, OR = 8.070, 95% CI: 1.018–63.997, respectively). Our results indicated that TAP and HLA-I may exert a combined effect on CHC susceptibility in the Chinese Han population.</p>","PeriodicalId":14003,"journal":{"name":"International Journal of Immunogenetics","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2022-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Association study of TAP and HLA-I gene combination with chronic hepatitis C virus infection in a Han population in China\",\"authors\":\"Yufen Tao, Xue Han, Nannan Liu, Lei Shi, Li Shi, Shuyuan Liu, Yufeng Yao\",\"doi\":\"10.1111/iji.12574\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Host immune system genes play key roles in the progression of chronic hepatitis C virus (HCV) infection. Transporters associated with antigen processing (TAP) play an important role in the loading of viral peptides onto MHC class I molecules. This study aimed to investigate the association between <i>TAP</i> gene polymorphisms and chronic HCV in a Chinese Han population. A total of 232 chronic hepatitis C (CHC) patients and 362 healthy individuals were recruited from the Han population in Yunnan province in southwest China, and a TaqMan SNP genotyping assay was used to detect six single nucleotide polymorphisms (SNPs) of <i>TAP1</i> and three SNPs of <i>TAP2</i> genes. The association of the <i>TAP</i> gene with CHC was analysed at the allele, genotype, and haplotype levels. There were no significant differences in the allele and genotype frequencies of these SNPs in the <i>TAP</i> gene between CHC patients and controls after Bonferroni correction. A novel <i>TAP1</i> allele (<i>TAP1*unknown_1</i>: rs41555220-rs41549617-rs1057141-rs1135216-rs1057149-rs41551515: G-G-A-G-G-G) was only present in the CHC group, and this allele significantly increased susceptibility to CHC (<i>p </i>= .005, odds ratio [OR] = 11.105. 95% confidence interval [CI]: 1.362–90.558). Homozygous <i>TAP1*03:01/TAP1*03:01</i> was observed only in the CHC group that exhibited an obvious risk for CHC (<i>p </i>= .002, OR = 9.637, 95% CI: 1.153–80.574). And the haplotype <i>TAP1*unknown_1-TAP2*01:01</i> was only present in the CHC group and indicated a significant risk for CHC (<i>p </i>= .002, OR = 9.498, 95% CI: 1.140–79.149). We observed significant interactions among <i>HLA-A</i>, <i>-B</i>,<i>C</i>, <i>TAP1</i>, and <i>TAP2</i> alleles, and combination analysis revealed that the combination of <i>TAP1*01:01-TAP2*01:01-HLA-B*35:01</i> was only present in the control group (2.2%) and resulted in significantly increased resistance to CHC (<i>p </i>= .002, OR = 0.096, 95% CI: 0.012–0.759). Whereas, the combination of <i>TAP1*01:01-TAP2*01:01-HLA-C*07:02</i> and <i>TAP1*03:01-TAP2*01:01-HLA-C*01:02</i> increased the susceptibility to CHC significantly (<i>p </i>= .001, OR = 2.016, 95% CI: 1.309–3.106 and <i>p </i>= .002, OR = 8.070, 95% CI: 1.018–63.997, respectively). Our results indicated that TAP and HLA-I may exert a combined effect on CHC susceptibility in the Chinese Han population.</p>\",\"PeriodicalId\":14003,\"journal\":{\"name\":\"International Journal of Immunogenetics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2022-04-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Immunogenetics\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/iji.12574\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Immunogenetics","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/iji.12574","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
Association study of TAP and HLA-I gene combination with chronic hepatitis C virus infection in a Han population in China
Host immune system genes play key roles in the progression of chronic hepatitis C virus (HCV) infection. Transporters associated with antigen processing (TAP) play an important role in the loading of viral peptides onto MHC class I molecules. This study aimed to investigate the association between TAP gene polymorphisms and chronic HCV in a Chinese Han population. A total of 232 chronic hepatitis C (CHC) patients and 362 healthy individuals were recruited from the Han population in Yunnan province in southwest China, and a TaqMan SNP genotyping assay was used to detect six single nucleotide polymorphisms (SNPs) of TAP1 and three SNPs of TAP2 genes. The association of the TAP gene with CHC was analysed at the allele, genotype, and haplotype levels. There were no significant differences in the allele and genotype frequencies of these SNPs in the TAP gene between CHC patients and controls after Bonferroni correction. A novel TAP1 allele (TAP1*unknown_1: rs41555220-rs41549617-rs1057141-rs1135216-rs1057149-rs41551515: G-G-A-G-G-G) was only present in the CHC group, and this allele significantly increased susceptibility to CHC (p = .005, odds ratio [OR] = 11.105. 95% confidence interval [CI]: 1.362–90.558). Homozygous TAP1*03:01/TAP1*03:01 was observed only in the CHC group that exhibited an obvious risk for CHC (p = .002, OR = 9.637, 95% CI: 1.153–80.574). And the haplotype TAP1*unknown_1-TAP2*01:01 was only present in the CHC group and indicated a significant risk for CHC (p = .002, OR = 9.498, 95% CI: 1.140–79.149). We observed significant interactions among HLA-A, -B,C, TAP1, and TAP2 alleles, and combination analysis revealed that the combination of TAP1*01:01-TAP2*01:01-HLA-B*35:01 was only present in the control group (2.2%) and resulted in significantly increased resistance to CHC (p = .002, OR = 0.096, 95% CI: 0.012–0.759). Whereas, the combination of TAP1*01:01-TAP2*01:01-HLA-C*07:02 and TAP1*03:01-TAP2*01:01-HLA-C*01:02 increased the susceptibility to CHC significantly (p = .001, OR = 2.016, 95% CI: 1.309–3.106 and p = .002, OR = 8.070, 95% CI: 1.018–63.997, respectively). Our results indicated that TAP and HLA-I may exert a combined effect on CHC susceptibility in the Chinese Han population.
期刊介绍:
The International Journal of Immunogenetics (formerly European Journal of Immunogenetics) publishes original contributions on the genetic control of components of the immune system and their interactions in both humans and experimental animals. The term ''genetic'' is taken in its broadest sense to include studies at the evolutionary, molecular, chromosomal functional and population levels in both health and disease. Examples are:
-studies of blood groups and other surface antigens-
cell interactions and immune response-
receptors, antibodies, complement components and cytokines-
polymorphism-
evolution of the organisation, control and function of immune system components-
anthropology and disease associations-
the genetics of immune-related disease: allergy, autoimmunity, immunodeficiency and other immune pathologies-
All papers are seen by at least two independent referees and only papers of the highest quality are accepted.