{"title":"蝇科（直翅目）EST SSR的开发、鉴定及其跨属可转移性","authors":"Dan Wang, D. Yang","doi":"10.33338/EF.84682","DOIUrl":null,"url":null,"abstract":"EST-SSR (Expressed Sequence Tag-Simple Sequence Repeat)markers were developed and used to examine genetic diversity of species assemblages of ten genera of Muscidae (Diptera), and specifically the genetic diversity of Phaonia Robineau species, from ten regions in China. 18 EST-SSR markers with high polymorphism and clear bands were screened out from 216 tested markers, with 219 alleles in total (95–280 bp) with an average of 6.1 alleles per locus. In various regions in China such as Tibet, Sichuan, and Yunnan, Muscidae species assemblages exhibited rich genetic diversity, with the polymorphism information content (PIC) values ranging from 0.831 to 0.934, while Hainan region showed a relatively low genetic diversity (PIC = 0.511). Low gene flow (Nm = 0.842) was found among the regions, and the average genetic differentiation coefficient (Fst) was 0.238, indicating a high degree of genetic differentiation among the ten surveyed assemblages. Accordingly, the ten genera of Muscidae exhibited high genetic diversity and genetic differentiation.","PeriodicalId":50520,"journal":{"name":"Entomologica Fennica","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Development and characterization of EST-SSRs for Muscidae (Diptera) and their cross-genera transferability\",\"authors\":\"Dan Wang, D. Yang\",\"doi\":\"10.33338/EF.84682\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"EST-SSR (Expressed Sequence Tag-Simple Sequence Repeat)markers were developed and used to examine genetic diversity of species assemblages of ten genera of Muscidae (Diptera), and specifically the genetic diversity of Phaonia Robineau species, from ten regions in China. 18 EST-SSR markers with high polymorphism and clear bands were screened out from 216 tested markers, with 219 alleles in total (95–280 bp) with an average of 6.1 alleles per locus. In various regions in China such as Tibet, Sichuan, and Yunnan, Muscidae species assemblages exhibited rich genetic diversity, with the polymorphism information content (PIC) values ranging from 0.831 to 0.934, while Hainan region showed a relatively low genetic diversity (PIC = 0.511). Low gene flow (Nm = 0.842) was found among the regions, and the average genetic differentiation coefficient (Fst) was 0.238, indicating a high degree of genetic differentiation among the ten surveyed assemblages. Accordingly, the ten genera of Muscidae exhibited high genetic diversity and genetic differentiation.\",\"PeriodicalId\":50520,\"journal\":{\"name\":\"Entomologica Fennica\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-08-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Entomologica Fennica\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.33338/EF.84682\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Entomologica Fennica","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.33338/EF.84682","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 2
摘要
利用EST-SSR（Expressed Sequence Tag Simple Sequence Repeat，表达序列标签-重复序列）标记对我国十个地区蝇科十属物种组合的遗传多样性进行了研究，特别是对斑蝶属物种的遗传多样度进行了研究。从216个待测标记中筛选出18个具有高度多态性和清晰条带的EST-SSR标记，共有219个等位基因（95-280bp），平均每个位点有6.1个等位位点。在西藏、四川和云南等中国各地区，蝇科物种组合表现出丰富的遗传多样性，多态性信息含量（PIC）值在0.831至0.934之间，而海南地区则表现出相对较低的遗传多样度（PIC=0.511），平均遗传分化系数（Fst）为0.238，表明10个调查组合的遗传分化程度较高。因此，蝇科的10个属表现出较高的遗传多样性和遗传分化。
Development and characterization of EST-SSRs for Muscidae (Diptera) and their cross-genera transferability
EST-SSR (Expressed Sequence Tag-Simple Sequence Repeat)markers were developed and used to examine genetic diversity of species assemblages of ten genera of Muscidae (Diptera), and specifically the genetic diversity of Phaonia Robineau species, from ten regions in China. 18 EST-SSR markers with high polymorphism and clear bands were screened out from 216 tested markers, with 219 alleles in total (95–280 bp) with an average of 6.1 alleles per locus. In various regions in China such as Tibet, Sichuan, and Yunnan, Muscidae species assemblages exhibited rich genetic diversity, with the polymorphism information content (PIC) values ranging from 0.831 to 0.934, while Hainan region showed a relatively low genetic diversity (PIC = 0.511). Low gene flow (Nm = 0.842) was found among the regions, and the average genetic differentiation coefficient (Fst) was 0.238, indicating a high degree of genetic differentiation among the ten surveyed assemblages. Accordingly, the ten genera of Muscidae exhibited high genetic diversity and genetic differentiation.
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