A. Sekenova, Yelena Li, A. Saparov, B. Mambetpayeva, N. Kulmaganbetova, Y. Ramankulov, V. Ogay
{"title":"细胞因子预处理致密骨源间充质干细胞在二维和三维培养条件下的免疫调节特性","authors":"A. Sekenova, Yelena Li, A. Saparov, B. Mambetpayeva, N. Kulmaganbetova, Y. Ramankulov, V. Ogay","doi":"10.26577/ijbch.2021.v14.i2.04","DOIUrl":null,"url":null,"abstract":"em cells (MSCs) possess potent immunomodulatory properties and therefore represent a promising therapeutic tool for the treatment of immune-related diseases. Recently, preconditioning has been proposed as a rategy to improve the immunomodulatory activity of MSCs. In this study, we focused to inve on immunomodulatory activity of mouse compact bone-derived MSCs (CB MSCs) in 2D and 3D culture conditions. Mouse CB MSCs for the 2D condition were cultured in a andard tissue culture plate. 3D spheroid MSCs were formed by enforced composing in Nunclon Sphera 96-well U-bottom plate. MSCs in 2D and levels in lymphocytes and macrophages respectively. It was found that 3D spheroid MSCs more effectively suppressed the proliferation of T cells compared with 2D MSCs culture. Moreover, preconditioning of 3D that preconditioning with pro-compared to 2D MSC culture.","PeriodicalId":41021,"journal":{"name":"International Journal of Biology and Chemistry","volume":" ","pages":""},"PeriodicalIF":0.3000,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Immunomodulatory Properties of Cytokine-preconditioned Compact-bone Derived Mesenchymal Stem Cells Cultured in 2D and 3D Culture Conditions\",\"authors\":\"A. Sekenova, Yelena Li, A. Saparov, B. Mambetpayeva, N. Kulmaganbetova, Y. Ramankulov, V. Ogay\",\"doi\":\"10.26577/ijbch.2021.v14.i2.04\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"em cells (MSCs) possess potent immunomodulatory properties and therefore represent a promising therapeutic tool for the treatment of immune-related diseases. Recently, preconditioning has been proposed as a rategy to improve the immunomodulatory activity of MSCs. In this study, we focused to inve on immunomodulatory activity of mouse compact bone-derived MSCs (CB MSCs) in 2D and 3D culture conditions. Mouse CB MSCs for the 2D condition were cultured in a andard tissue culture plate. 3D spheroid MSCs were formed by enforced composing in Nunclon Sphera 96-well U-bottom plate. MSCs in 2D and levels in lymphocytes and macrophages respectively. It was found that 3D spheroid MSCs more effectively suppressed the proliferation of T cells compared with 2D MSCs culture. Moreover, preconditioning of 3D that preconditioning with pro-compared to 2D MSC culture.\",\"PeriodicalId\":41021,\"journal\":{\"name\":\"International Journal of Biology and Chemistry\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.3000,\"publicationDate\":\"2021-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Biology and Chemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.26577/ijbch.2021.v14.i2.04\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Biology and Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.26577/ijbch.2021.v14.i2.04","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOLOGY","Score":null,"Total":0}
Immunomodulatory Properties of Cytokine-preconditioned Compact-bone Derived Mesenchymal Stem Cells Cultured in 2D and 3D Culture Conditions
em cells (MSCs) possess potent immunomodulatory properties and therefore represent a promising therapeutic tool for the treatment of immune-related diseases. Recently, preconditioning has been proposed as a rategy to improve the immunomodulatory activity of MSCs. In this study, we focused to inve on immunomodulatory activity of mouse compact bone-derived MSCs (CB MSCs) in 2D and 3D culture conditions. Mouse CB MSCs for the 2D condition were cultured in a andard tissue culture plate. 3D spheroid MSCs were formed by enforced composing in Nunclon Sphera 96-well U-bottom plate. MSCs in 2D and levels in lymphocytes and macrophages respectively. It was found that 3D spheroid MSCs more effectively suppressed the proliferation of T cells compared with 2D MSCs culture. Moreover, preconditioning of 3D that preconditioning with pro-compared to 2D MSC culture.
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