儿童T淋巴细胞相关细胞因子与慢性免疫性血小板减少症预后的相关性研究

Q4 Medicine 中华实用儿科临床杂志 Pub Date : 2020-03-19 DOI:10.3760/CMA.J.CN101070-20190527-00454

Pei-ling Li, Xinxin Wang, Rui Fan, Bao Liu, Aiju Xiao, Yanhua Wang, Dongju Zhao, Tai-xin Shi

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引用次数: 0

摘要

目的检测慢性免疫性血小板减少症(ITP)患儿外周血细胞因子水平，分析其对慢性ITP患儿临床预后的影响。方法选取2015年10月至2018年10月在新乡医科大学附属第一医院儿科就诊的30例慢性ITP患儿作为实验组，选取40例同一医院的健康患儿作为健康对照组。采用流式细胞术(CBA)检测实验组和健康对照组白细胞介素-2(IL-2)、干扰素-γ(IFN-γ)、肿瘤坏死因子(TNF)、白细胞介素-4(IL-4)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、白细胞介素- 17a (IL-17A)水平。分析细胞因子与慢性ITP患儿预后的关系。结果入选ITP患者30例。实验组治疗前IL-2、IL-17A表达量分别为(7.86±3.90)ng/L和(10.45±12.35)ng/L，健康对照组IL-2、IL-17A表达量分别为(3.11±2.41)ng/L和(2.97±7.04)ng/L。实验组患者IL-2、IL-17A水平显著高于健康对照组，差异均有统计学意义(t=-7.123、-5.582,P均0.05);慢性ITP患者治疗前与治疗后12个月细胞因子水平差异无统计学意义(P < 0.05)。结论T淋巴细胞相关细胞因子的变化与儿童慢性ITP的发病发展密切相关。慢性ITP患儿可能存在持续的免疫功能障碍。动态监测IL-2、IL-4、IL-10、IL-17A，尤其是IL-17A，有助于判断儿童ITP的预后，对评估临床预后可能具有指导意义。关键词:慢性;免疫性血小板减少症;T淋巴细胞相关细胞因子;结果;孩子

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Correlation study between T lymphocyte-related cytokines and the outcome of chronic immune thrombocytopenia in children

Objective To detect the levels of cytokines in peripheral blood of patients with chronic immune thrombocytopenia (ITP) and analyze their significance in the clinical prognosis of children with chronic ITP. Methods Thirty patients with chronic ITP who were treated in the Department of Pediatrics, the First Affiliated Hospital of Xinxiang Medical Univercity from October 2015 to October 2018 were followed and enrolled in the experimental group and 40 healthy children in the same hospital were enrolled in the healthy control group.The levels of interleukin-2(IL-2), interferon-γ(IFN-γ), tumor necrosis factor(TNF), interleukin-4(IL-4), interleukin-6(IL-6), interleukin-10(IL-10) and interleukin-17A(IL-17A) in the experimental group and the healthy control group were detected by flow cytometry (CBA). The relationship between cytokines and prognosis of children with chronic ITP were analyzed. Results Thirty patients with ITP were enrolled. The expressions of IL-2 and IL-17A in the experimental group before treatment were (7.86±3.90) ng/L and (10.45±12.35) ng/L, while those of IL-2 and IL-17A in the healthy control group were (3.11±2.41) ng/L and (2.97±7.04) ng/L. The levels of IL-2 and IL-17A in the experimental group were significantly higher than those in the healthy control group, and the differences were statistically significant (t=-7.123, -5.582, all P 0.05), and no significant difference in the levels of cytokines between the patients with chronic ITP before and 12 months after treatment (P>0.05). Conclusions The changes of T lymphocyte related cytokines are closely related to the pathogenesis and development of chronic ITP in children. There may be persistent immune dysfunction in children with chronic ITP. Dynamic monitoring of cytokines IL-2, IL-4, IL-10, IL-17A, especially IL-17A, is helpful to judge the prognosis of ITP in children, and may be of guiding significance in evaluating clinical prognosis. Key words: Chronic; Immune thrombocytopenia; T lymphocyte-related cytokines; Outcome; Child

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来源期刊

中华实用儿科临床杂志 Medicine-Pediatrics, Perinatology and Child Health

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0.60

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0.00%

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14243

期刊介绍： Chinese Journal of Applied Clinical Pediatrics ( semi-monthly ) is a core journal of paediatrics under the supervision of China Association for Science and Technology, sponsored by Chinese Medical Association and undertaken by Xinxiang Medical College. Founded in 1986, it is openly circulated both at home and abroad. The journal has several columns, such as Expert Forum, Experimental Research and Paediatric Surgery, which are mainly for paediatric medical workers and medical researchers in hospitals. Its purpose is to reflect the new theories and technologies in paediatric medicine and scientific research at home and abroad, and to promote academic exchanges. Chinese Journal of Applied Clinical Pediatrics is a source journal of China Science Citation Database (CSCD), a core journal of Peking University, a source journal of Chinese science and technology paper statistics (China Science and Technology Core Journals), a core academic journal of RCCSE, a high-quality scientific and technical journal of China, a high-quality scientific and technical journal of China Association for Science and Technology, and a high-quality scientific and technical journal of China Biomedical Science and Technology Association. We have been published in China Biomedical Literature Database (SinoMed), China Knowledge Network, Wanfang Data Knowledge Service Platform, China Academic Journal Abstracts, Scopus Database, Chemical Abstracts (USA), Japan Science and Technology Agency (JSTA) Database, Copernicus Abstracts (Poland), Abstracts of the Centre for Agricultural and Biological Sciences (CABS) of the United Kingdom, Cambridge Scientific Abstracts ProQuest Database, WHO Medical Journal of the Western Pacific Region (WMPR), and WHO Medical Journal of the Western Pacific Region (WMPR) of the United States. We have been included in dozens of authoritative databases at home and abroad, such as WHO Western Pacific Region Index of Medicine (WPRIM), Ullrich's Guide to Periodicals, and so on.