急性白血病伴或不伴RUNX1突变的浆细胞样树突状细胞CD123+区室:免疫表型无监督分析和聚类揭示的高患者间变异性

IF 0.9 Q4 HEMATOLOGY Hemato Pub Date : 2021-09-01 DOI:10.3390/hemato2030036
A. Porwit, M. Béné
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引用次数: 2

摘要

浆细胞样树突状细胞(PDC)是正常骨髓(BM)细胞的一小部分,但也已被证明存在于几种血液系统恶性肿瘤中,有时大量存在,如RUNX1突变的急性髓系白血病、慢性粒单核细胞白血病,或明显的成母细胞浆细胞样树突细胞肿瘤。据报道,这些细胞在不同条件下表现出一些可变的免疫表型特征。然而,人们对其在个体患者中的可塑性知之甚少。使用无监督聚类工具(FlowSOM)重新访问先前分析的7例血液系统恶性肿瘤(6例急性粒细胞白血病和1例慢性粒单核细胞白血病)的流式细胞术结果,我们在这里报告了PDC亚群出乎意料的高变异性。尽管五名研究患者携带RUNX1突变,但没有发现PDCs的一致特征与该变体有关。此外,在六个正常BM的合并文件中检测到的PDC的一个正常单节点小子集可以在三个成功治疗的患者的缓解BM样本中检索到。这项研究强调了无监督流式细胞术分析描绘经典监督工具无法检测到的细胞亚群的能力。
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The Plasmacytoid Dendritic Cell CD123+ Compartment in Acute Leukemia with or without RUNX1 Mutation: High Inter-Patient Variability Disclosed by Immunophenotypic Unsupervised Analysis and Clustering
Plasmacytoid dendritic cells (PDC) constitute a small subset of normal bone marrow (BM) cells but have also been shown to be present, sometimes in large numbers, in several hematological malignancies such as acute myeloid leukemia with RUNX1 mutation, chronic myelomonocytic leukemia or, obviously, blastic plasmacytoid dendritic cell neoplasms. These cells have been reported to display somewhat variable immunophenotypic features in different conditions. However, little is known of their plasticity within individual patients. Using an unsupervised clustering tool (FlowSOM) to re-visit flow cytometry results of seven previously analyzed cases of hematological malignancies (6 acute myeloid leukemia and one chronic myelomonocytic leukemia) with a PDC contingent, we report here on the unexpectedly high variability of PDC subsets. Although five of the studied patients harbored a RUNX1 mutation, no consistent feature of PDCs could be disclosed as associated with this variant. Moreover, the one normal single-node small subset of PDC detected in the merged file of six normal BM could be retrieved in the remission BM samples of three successfully treated patients. This study highlights the capacity of unsupervised flow cytometry analysis to delineate cell subsets not detectable with classical supervised tools.
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CiteScore
1.30
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审稿时长
11 weeks
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