上皮性卵巢癌DLX2高表达与不良预后及细胞增殖的相关性探讨

IF 0.5 4区 医学 Q4 OBSTETRICS & GYNECOLOGY European journal of gynaecological oncology Pub Date : 2021-06-15 DOI:10.31083/J.EJGO.2021.03.2093
Menghui Huang, Yu-Wen Han, Qinghua Xi, Yunfeng Jin, Ying-lei Liu, Yunhe Han, Hai-Li Kai, Qian Zhang, Yan-Li Zheng
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引用次数: 0

摘要

目的:研究发现远端少同源盒2(DLX2)的过度表达与多种癌症密切相关。然而,DLX2在卵巢癌发病机制中的作用尚不清楚。本研究旨在探讨DLX2对卵巢上皮癌细胞增殖和凋亡的作用机制。方法:对119例EOC组织标本进行免疫组化分析,确定DLX2表达水平与临床病理特征的相关性。此外,采用蛋白质印迹法测定了不同级别EOC标本和EOC细胞系中DLX2蛋白的表达,并探讨了其作用的分子机制。Kaplan-Meier生存分析显示,DLX2的过度表达与EOC的不良临床结果明显相关(P<0.01)。使用shRNA-DLX2敲除DLX2降低了EOC细胞的增殖并增强了细胞凋亡。结论:DLX2可能是一种新的预后指标,对EOCs的分子靶向治疗药物具有重要价值。
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Exploration on correlation of high DLX2 expression with poor prognosis and cellular proliferation in epithelial ovarian cancers
Objective: It has been found that overexpression of distal-less homeobox 2 (DLX2) is closely correlated with multiple cancers. However, the role of DLX2 in the pathogenesis of ovarian cancers is not known. This study was designed to explore the mechanism of action of DLX2 on cellular proliferation and apoptosis in epithelial ovarian cancers (EOCs). Methods: A total of 119 EOC tissue specimens were analyzed immunohistochemically, and the correlation between DLX2 expressional level and clinicopathological characteristics was determined. Moreover, western blot method was used to measure DLX2 protein in EOC specimen of different grades and EOC cell lines and explore its molecular mechanism of action. Kaplan-Meier survival analysis revealed that overexpression of DLX2 was obviously correlated with an adverse clinical outcome in EOCs (P < 0.01*). Results: DLX2 expressional level had an obvious association with histopathological grade, FIGO stage, ascites and ki-67 expressional level in EOC patients and DLX2 exerted a crucial effect in regulating cellular proliferation in EOCs. Knocking down DLX2 using shRNA-DLX2 reduced the proliferation and enhanced the apoptosis in EOC cells. Conclusion: DLX2 might act as a new prognostic indicator and have an important value for molecular targeted therapeutic drugs in EOCs.
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来源期刊
自引率
25.00%
发文量
58
审稿时长
1 months
期刊介绍: EJGO is dedicated to publishing editorial articles in the Distinguished Expert Series and original research papers, case reports, letters to the Editor, book reviews, and newsletters. The Journal was founded in 1980 the second gynaecologic oncology hyperspecialization Journal in the world. Its aim is the diffusion of scientific, clinical and practical progress, and knowledge in female neoplastic diseases in an interdisciplinary approach among gynaecologists, oncologists, radiotherapists, surgeons, chemotherapists, pathologists, epidemiologists, and so on.
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