Joadil G. Abreu, Víctor M. Fernández-Cabanás, Janderson A. Rodrigues, Matheus Abreu, Eduardo A. Ferreira, Wender M. Peixoto
{"title":"近红外光谱法测定象草的体外消化率和发酵率","authors":"Joadil G. Abreu, Víctor M. Fernández-Cabanás, Janderson A. Rodrigues, Matheus Abreu, Eduardo A. Ferreira, Wender M. Peixoto","doi":"10.1111/grs.12383","DOIUrl":null,"url":null,"abstract":"<p>The methods used to evaluate kinetic parameters of ruminant feeds can be in situ and in vitro. For both methods, it is necessary to maintain cannulated animals in order to collect the inoculum from the rumen, which has been under strong pressure from society, in terms of animal welfare policies, to avoid this type of practices. This work aimed to evaluate the estimation of in vitro digestibility and fermentation of elephant grass forage by near infrared reflectance spectroscopy (NIRS), as a fast and noninvasive alternative to the in vitro method. The experimental design was in randomized blocks with three repetitions. The treatments were arranged in a split-plot scheme, with 13 genotypes in the plot and 15 regrowth ages in the subplot. Crude protein (CP) and neutral detergent fiber (NDF) contents; in vitro dry matter (IVDMD) and NDF (IVNDFD) digestibilities; and total volume (V<sub>t</sub>), gas production rate (μ) and half-life conventional analyses were determined for pre-dried elephant grass forage samples. For the evaluation of NIRS models performance, coefficient of determination (<i>R</i><sup>2</sup>cv) and standard error of cross validation (SECV) were used. As the regrowth age advanced, there was a linear increase in the NDF content and a linear reduction in the IVDMD, IVNDFD and V<sub>t</sub> values. The NIRS estimates proved to be adequate for IVDMD (<i>R</i><sup>2</sup>cv = .95), IVNDFD (<i>R</i><sup>2</sup>cv = .85), V<sub>t</sub> (<i>R</i><sup>2</sup>cv = .81) and gas production at 48 (<i>R</i><sup>2</sup>cv = .82), 72 (<i>R</i><sup>2</sup>cv = .85) and 96 h (<i>R</i><sup>2</sup>cv = .84), confirming as an alternative to the in vitro methods that dispense with the use of cannulated animals.</p>","PeriodicalId":56078,"journal":{"name":"Grassland Science","volume":"69 1","pages":"33-41"},"PeriodicalIF":1.1000,"publicationDate":"2022-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Estimation of in vitro digestibility and fermentation of elephant grass by near infrared spectroscopy\",\"authors\":\"Joadil G. Abreu, Víctor M. Fernández-Cabanás, Janderson A. Rodrigues, Matheus Abreu, Eduardo A. Ferreira, Wender M. Peixoto\",\"doi\":\"10.1111/grs.12383\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The methods used to evaluate kinetic parameters of ruminant feeds can be in situ and in vitro. For both methods, it is necessary to maintain cannulated animals in order to collect the inoculum from the rumen, which has been under strong pressure from society, in terms of animal welfare policies, to avoid this type of practices. This work aimed to evaluate the estimation of in vitro digestibility and fermentation of elephant grass forage by near infrared reflectance spectroscopy (NIRS), as a fast and noninvasive alternative to the in vitro method. The experimental design was in randomized blocks with three repetitions. The treatments were arranged in a split-plot scheme, with 13 genotypes in the plot and 15 regrowth ages in the subplot. Crude protein (CP) and neutral detergent fiber (NDF) contents; in vitro dry matter (IVDMD) and NDF (IVNDFD) digestibilities; and total volume (V<sub>t</sub>), gas production rate (μ) and half-life conventional analyses were determined for pre-dried elephant grass forage samples. For the evaluation of NIRS models performance, coefficient of determination (<i>R</i><sup>2</sup>cv) and standard error of cross validation (SECV) were used. As the regrowth age advanced, there was a linear increase in the NDF content and a linear reduction in the IVDMD, IVNDFD and V<sub>t</sub> values. The NIRS estimates proved to be adequate for IVDMD (<i>R</i><sup>2</sup>cv = .95), IVNDFD (<i>R</i><sup>2</sup>cv = .85), V<sub>t</sub> (<i>R</i><sup>2</sup>cv = .81) and gas production at 48 (<i>R</i><sup>2</sup>cv = .82), 72 (<i>R</i><sup>2</sup>cv = .85) and 96 h (<i>R</i><sup>2</sup>cv = .84), confirming as an alternative to the in vitro methods that dispense with the use of cannulated animals.</p>\",\"PeriodicalId\":56078,\"journal\":{\"name\":\"Grassland Science\",\"volume\":\"69 1\",\"pages\":\"33-41\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2022-09-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Grassland Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/grs.12383\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Grassland Science","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/grs.12383","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
Estimation of in vitro digestibility and fermentation of elephant grass by near infrared spectroscopy
The methods used to evaluate kinetic parameters of ruminant feeds can be in situ and in vitro. For both methods, it is necessary to maintain cannulated animals in order to collect the inoculum from the rumen, which has been under strong pressure from society, in terms of animal welfare policies, to avoid this type of practices. This work aimed to evaluate the estimation of in vitro digestibility and fermentation of elephant grass forage by near infrared reflectance spectroscopy (NIRS), as a fast and noninvasive alternative to the in vitro method. The experimental design was in randomized blocks with three repetitions. The treatments were arranged in a split-plot scheme, with 13 genotypes in the plot and 15 regrowth ages in the subplot. Crude protein (CP) and neutral detergent fiber (NDF) contents; in vitro dry matter (IVDMD) and NDF (IVNDFD) digestibilities; and total volume (Vt), gas production rate (μ) and half-life conventional analyses were determined for pre-dried elephant grass forage samples. For the evaluation of NIRS models performance, coefficient of determination (R2cv) and standard error of cross validation (SECV) were used. As the regrowth age advanced, there was a linear increase in the NDF content and a linear reduction in the IVDMD, IVNDFD and Vt values. The NIRS estimates proved to be adequate for IVDMD (R2cv = .95), IVNDFD (R2cv = .85), Vt (R2cv = .81) and gas production at 48 (R2cv = .82), 72 (R2cv = .85) and 96 h (R2cv = .84), confirming as an alternative to the in vitro methods that dispense with the use of cannulated animals.
Grassland ScienceAgricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
2.70
自引率
7.70%
发文量
38
审稿时长
>12 weeks
期刊介绍:
Grassland Science is the official English language journal of the Japanese Society of Grassland Science. It publishes original research papers, review articles and short reports in all aspects of grassland science, with an aim of presenting and sharing knowledge, ideas and philosophies on better management and use of grasslands, forage crops and turf plants for both agricultural and non-agricultural purposes across the world. Contributions from anyone, non-members as well as members, are welcome in any of the following fields:
grassland environment, landscape, ecology and systems analysis;
pasture and lawn establishment, management and cultivation;
grassland utilization, animal management, behavior, nutrition and production;
forage conservation, processing, storage, utilization and nutritive value;
physiology, morphology, pathology and entomology of plants;
breeding and genetics;
physicochemical property of soil, soil animals and microorganisms and plant
nutrition;
economics in grassland systems.