RP-HPLC同时测定胶囊中甲硝唑、呋喃唑酮和二环胺的含量

IF 0.7 Q4 PHARMACOLOGY & PHARMACY Journal of Reports in Pharmaceutical Sciences Pub Date : 2021-01-01 DOI:10.4103/jrptps.JRPTPS_104_19
Amuthalakshmi Sivaperuman, N. Nagarajan, Uma Jayapaul
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引用次数: 1

摘要

首次建立了一种新的、简单、准确的反相高效液相色谱法,用Thermo,C8(150mm×4.6)柱同时测定胶囊剂型中甲硝唑、呋喃唑酮和盐酸二环胺的含量。最佳分离是通过乙腈、水(40:60)和含10%w/v氢氧化钠(pH 7.5)的20mm磷酸盐缓冲液的流动相梯度洗脱实现的,检测波长为215nm。分离在运行时间的15分钟内完成。甲硝唑、呋喃唑酮和盐酸二环胺的保留时间分别为1.79、2.45和11.50分钟。所提出的方法是线性的。根据ICH指南对该方法进行了统计验证,结果表明,该方法简单、准确、准确、线性,甲硝唑、呋喃唑酮和二环胺的重现性分别在40.2–60、40.2–60.4和3-5µg/mL范围内。所开发的方法首次预测了该方法在商业上可获得的剂型中同时估计三种药物的适用性。
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Simultaneous RP-HPLC estimation and validation of metronidazole, furazolidone, and dicyclomine in capsule
For the first time, a new, simple, precise reversed-phase high-performance liquid chromatography method was developed for the simultaneous estimation of metronidazole, furazolidone, and dicyclomine hydrochloride in capsule dosage form. The method was performed with Thermo, C8 (150 mm×4.6) column. The best separation was achieved by gradient elution with mobile phase of acetonitrile, water (40:60), and 20 mm phosphate buffer with 10% w/v sodium hydroxide (pH 7.5) with a detection wavelength of 215 nm. The separation was completed within 15 min of runtime. The retention time of metronidazole, furazolidone, and dicyclomine hydrochloride was found to be 1.79, 2.45, and 11.50 min, respectively. The proposed method was found to be linear. The method was statistically validated as per the ICH guidelines and shown to be simple, accurate, precise, linear, and reproducible in the range of 40.2–60, 40.2–60.4, and 3–5 µg/mL for metronidazole, furazolidone, and dicyclomine, respectively. For the first time, the developed method foretells the suitability of the method for the simultaneous estimation of three drugs in the commercially available dosage forms.
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来源期刊
Journal of Reports in Pharmaceutical Sciences
Journal of Reports in Pharmaceutical Sciences Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)
CiteScore
1.40
自引率
0.00%
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0
期刊介绍: The Journal of Reports in Pharmaceutical Sciences(JRPS) is a biannually peer-reviewed multi-disciplinary pharmaceutical publication to serve as a means for scientific information exchange in the international pharmaceutical forum. It accepts novel findings that contribute to advancement of scientific knowledge in pharmaceutical fields that not published or under consideration for publication anywhere else for publication in JRPS as original research article. all aspects of pharmaceutical sciences consist of medicinal chemistry, molecular modeling, drug design, pharmaceutics, biopharmacy, pharmaceutical nanotechnology, pharmacognosy, natural products, pharmaceutical biotechnology, pharmacology, toxicology and clinical pharmacy.
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