Pathogen-Imprinted Polymer Film Integrated probe/Ti3C2Tx MXenes Electrochemical Sensor for Highly Sensitive Determination of Listeria Monocytogenes

As one of the most hazardous and deadliest pathogens, Listeria monocytogenes (LM) posed various serious diseases to the human being, thus designing effective strategy for its detection is of great significance. In this work, by preparing Ti 3 C 2 T x MXenes nanoribbon (Ti 3 C 2 T x R) as carrier and selecting thionine (Th) acted simultaneously as signal probe and functional monomer, a LM pathogen-imprinted polymers (PIP) integrated probe electrochemical sensor was design to monitor LM for the first time, that was carried out through the electropolymerization of Th on the Ti 3 C 2 T x R/GCE surface in the exis-tence of LM. Upon eluting the templates from the LM imprinted cavities, the fabricated PIP/Ti 3 C 2 T x R/GCE sensor can rebound LM cells effectively. By recording the peak current of Th as the response signal, it can be weakened when LM cell was re-bound to the LM imprinted cavity on PIP/Ti 3 C 2 T x R/GCE, and the absolute values of peak current change increase with the increasement of LM concentrations. After optimizing three key parameters, a considerable low analytical limit (2 CFU mL -1 ) and wide linearity (10–10 8 CFU mL -1 ) for LM were achieved. In addition, the experiments demonstrated that the PIP/Ti 3 C 2 T x R sensor offers satisfactory selectivity, reproducibility and stability.