M. B. D. Silva, C. S. Aguiar, Cínthya Viana Souza Rocha, Sara Barbosa Santos, Matheus Santos dos Anjos, Rafael Sampaio Vieira Silva, P. B. Nishiyama, R. E. Fraga, M. A. Rocha, P. P. Oliveira
{"title":"甘油-果冻作为培养基，用于安装原生生物和轮虫的永久载玻片:用于教学目的的建议","authors":"M. B. D. Silva, C. S. Aguiar, Cínthya Viana Souza Rocha, Sara Barbosa Santos, Matheus Santos dos Anjos, Rafael Sampaio Vieira Silva, P. B. Nishiyama, R. E. Fraga, M. A. Rocha, P. P. Oliveira","doi":"10.5007/2175-7925.2020.e75404","DOIUrl":null,"url":null,"abstract":"The proposal of this study is a protocol for the preparation of permanent sheets of protists and rotifers by adapting a Kisser glyceryn-jelly technique in three stages. A total of 450 sheets of protists and rotifers were made. After a period of 2 years, the slides remained stable, with no signs of fungal contamination and the organisms maintained their integral morphological conformation. The proposed technique is promising for the conservation of the morphological characteristics of the specimens, presenting low risk of manipulation, low cost, few reagents and easy handling, allowing the creation of a didactic collection for practical classes.","PeriodicalId":29999,"journal":{"name":"Biotemas","volume":"1 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2020-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Glycerin-Jelly as medium mounting permanent slides of protists and rotifers: a proposal for didactic purposes\",\"authors\":\"M. B. D. Silva, C. S. Aguiar, Cínthya Viana Souza Rocha, Sara Barbosa Santos, Matheus Santos dos Anjos, Rafael Sampaio Vieira Silva, P. B. Nishiyama, R. E. Fraga, M. A. Rocha, P. P. Oliveira\",\"doi\":\"10.5007/2175-7925.2020.e75404\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The proposal of this study is a protocol for the preparation of permanent sheets of protists and rotifers by adapting a Kisser glyceryn-jelly technique in three stages. A total of 450 sheets of protists and rotifers were made. After a period of 2 years, the slides remained stable, with no signs of fungal contamination and the organisms maintained their integral morphological conformation. The proposed technique is promising for the conservation of the morphological characteristics of the specimens, presenting low risk of manipulation, low cost, few reagents and easy handling, allowing the creation of a didactic collection for practical classes.\",\"PeriodicalId\":29999,\"journal\":{\"name\":\"Biotemas\",\"volume\":\"1 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-12-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biotemas\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5007/2175-7925.2020.e75404\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotemas","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5007/2175-7925.2020.e75404","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Glycerin-Jelly as medium mounting permanent slides of protists and rotifers: a proposal for didactic purposes
The proposal of this study is a protocol for the preparation of permanent sheets of protists and rotifers by adapting a Kisser glyceryn-jelly technique in three stages. A total of 450 sheets of protists and rotifers were made. After a period of 2 years, the slides remained stable, with no signs of fungal contamination and the organisms maintained their integral morphological conformation. The proposed technique is promising for the conservation of the morphological characteristics of the specimens, presenting low risk of manipulation, low cost, few reagents and easy handling, allowing the creation of a didactic collection for practical classes.
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