MSMS辅助10MHz压电免疫传感器固定化牛血清白蛋白的原位硅预测

IF 2.4 Q3 MATERIALS SCIENCE, MULTIDISCIPLINARY Journal of Molecular and Engineering Materials Pub Date : 2019-09-23 DOI:10.1142/S2251237319500011
Kaory Barrientos Urdinola, Paula Andrea Marín Muñoz, Pedronel Araque Marín, Marisol Jaramillo Grajales
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引用次数: 2

摘要

压电换能器有源区上的生物传感接口负责这些设备的灵敏度、特异性、可重复使用性和再现性。在用于功能化压电换能器的方法中,混合自组装单层(MSAM)是最成功的方法之一,因为它们可以获得半结晶分子阵列并在表面上布置生物受体。但是,要在基质上有效地部署MSAM,必须优化和表征它们与生物受体之间的结构比例。在本文中,我们建立了牛血清白蛋白(BSA)和MSAM功能化金基质之间相互作用的分子模型。首先,我们评估了底物功能化的条件,发现50:16巯基十六烷酸(MHDA)与11巯基-1-十一烷醇(MUA)的比例在表面上产生了最好的特征。我们还通过原子力显微镜(AFM),然后在10[公式:见正文]MHz石英晶体微天平(QCM)上,评估了在MSAM上固定BSA的具体条件(使用上述比率),并且根据所获得的数据,我们得出结论,当使用1[式:见正文][式:见文本]M MHDA和200[式:看正文][式,见文本]g/mL BSA时,获得0.005的结构比(MSAM/BSA),为压电换能器的功能化提供了最合适的条件。
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In-Silico Prediction on the MSAMS-Assisted Immobilization of Bovine Serum Albumin on 10MHz Piezoelectric Immunosensors
The biological sensing interface on the active area of a piezo transducer is responsible for the sensitivity, specificity, reusability, and reproducibility of these devices. Among the approaches used to functionalize piezo transducers, mixed self-assembled monolayers (MSAMs) are one of the most successful, given that they allow the obtaining of semi-crystalline molecular arrays and the arrangement of a bioreceptor on the surface. But, to deploy MSAMs on a substrate effectively, one must optimize and characterize the structural ratio between them and the bioreceptor. In this paper, we developed a molecular model of the interaction between Bovine Serum Albumin (BSA) and MSAMs-functionalized gold substrates. First, we evaluated the conditions for the functionalization of the substrates and found that a 50:1 16-mercaptohexadecaonic acid (MHDA) to 11 mercapto-1-undecanol (MUA) ratio produced the best features on the surface. We also evaluated the specific conditions to immobilize BSA on MSAMs (using the afore-established ratio) via Atomic Force Microscopy (AFM), and then on a 10[Formula: see text]MHz quartz crystal microbalance (QCM), and with the data obtained we concluded that a structural ratio of 0.005 (MSAM/BSA) is obtained when 1[Formula: see text][Formula: see text]M MHDA and 200[Formula: see text][Formula: see text]g/mL BSA were used, provided the most suitable conditions for the functionalization of a piezo transducer.
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Journal of Molecular and Engineering Materials
Journal of Molecular and Engineering Materials MATERIALS SCIENCE, MULTIDISCIPLINARY-
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