{"title":"美国内布拉斯加州豆科小檗(pediomelum tenuflorum, Pursh) A.N. Egan)居群的分子和形态变异","authors":"C. Kellar, P. R. Kellar","doi":"10.32873/UNL.DC.TNAS.39.17","DOIUrl":null,"url":null,"abstract":"Individuals of Pediomelum tenuiflorum, “wild alfalfa”, from disjunct populations in Nebraska vary extensively in their overall gestalt.\nThose in the western and central part of the state have a very slender growth habit, with thin stems and few, small flowers; whereas,\nthose in the southeast have a very robust growth habit with heavy-looking stems and many tightly clustered flowers. For nearly 200\nyears, taxonomists have alternated between splitting P. tenuiflorum into two species, with the many-flowered morphotype named\nP. floribundum, and lumping all the morphological variants into one species as they are now. In this study, we investigated morphological\nand molecular characters that could be used to clarify taxonomic classifications of these morphotypes. We measured 10\nmorphological characters on 51 specimens and sequenced nearly 300,000 nucleotide characters on the Illumina platform from three\ncellular genomes in seven samples of Pediomelum plus an outgroup taxon. Results revealed six significantly different morphological\ncharacters but ambiguous evolutionary histories of the plastid and mitochondrial genomes in P. tenuiflorum. Our complete plastid\ngenomes and genes and noncoding regions of the mitochondrial genome may be used as a foundation for studying the evolutionary\nhistories of these genomes. Additionally, we identified seven highly variable genomic regions in the chloroplast genome upon\nwhich a molecular phylogenetic investigation on an expanded set of samples from across the species’ geographic distribution can\nbe conducted to further define the taxonomic placements of P. tenuiflorum and P. floribundum.","PeriodicalId":91981,"journal":{"name":"Transactions of the Nebraska Academy of Sciences and affiliated societies","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular and Morphological Variation among Populations of\\nPediomelum tenuiflorum (Pursh) A.N. Egan (Fabaceae) in\\nNebraska, USA\",\"authors\":\"C. Kellar, P. R. Kellar\",\"doi\":\"10.32873/UNL.DC.TNAS.39.17\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Individuals of Pediomelum tenuiflorum, “wild alfalfa”, from disjunct populations in Nebraska vary extensively in their overall gestalt.\\nThose in the western and central part of the state have a very slender growth habit, with thin stems and few, small flowers; whereas,\\nthose in the southeast have a very robust growth habit with heavy-looking stems and many tightly clustered flowers. For nearly 200\\nyears, taxonomists have alternated between splitting P. tenuiflorum into two species, with the many-flowered morphotype named\\nP. floribundum, and lumping all the morphological variants into one species as they are now. In this study, we investigated morphological\\nand molecular characters that could be used to clarify taxonomic classifications of these morphotypes. We measured 10\\nmorphological characters on 51 specimens and sequenced nearly 300,000 nucleotide characters on the Illumina platform from three\\ncellular genomes in seven samples of Pediomelum plus an outgroup taxon. Results revealed six significantly different morphological\\ncharacters but ambiguous evolutionary histories of the plastid and mitochondrial genomes in P. tenuiflorum. Our complete plastid\\ngenomes and genes and noncoding regions of the mitochondrial genome may be used as a foundation for studying the evolutionary\\nhistories of these genomes. Additionally, we identified seven highly variable genomic regions in the chloroplast genome upon\\nwhich a molecular phylogenetic investigation on an expanded set of samples from across the species’ geographic distribution can\\nbe conducted to further define the taxonomic placements of P. tenuiflorum and P. floribundum.\",\"PeriodicalId\":91981,\"journal\":{\"name\":\"Transactions of the Nebraska Academy of Sciences and affiliated societies\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-07-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Transactions of the Nebraska Academy of Sciences and affiliated societies\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.32873/UNL.DC.TNAS.39.17\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Transactions of the Nebraska Academy of Sciences and affiliated societies","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.32873/UNL.DC.TNAS.39.17","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Molecular and Morphological Variation among Populations of
Pediomelum tenuiflorum (Pursh) A.N. Egan (Fabaceae) in
Nebraska, USA
Individuals of Pediomelum tenuiflorum, “wild alfalfa”, from disjunct populations in Nebraska vary extensively in their overall gestalt.
Those in the western and central part of the state have a very slender growth habit, with thin stems and few, small flowers; whereas,
those in the southeast have a very robust growth habit with heavy-looking stems and many tightly clustered flowers. For nearly 200
years, taxonomists have alternated between splitting P. tenuiflorum into two species, with the many-flowered morphotype named
P. floribundum, and lumping all the morphological variants into one species as they are now. In this study, we investigated morphological
and molecular characters that could be used to clarify taxonomic classifications of these morphotypes. We measured 10
morphological characters on 51 specimens and sequenced nearly 300,000 nucleotide characters on the Illumina platform from three
cellular genomes in seven samples of Pediomelum plus an outgroup taxon. Results revealed six significantly different morphological
characters but ambiguous evolutionary histories of the plastid and mitochondrial genomes in P. tenuiflorum. Our complete plastid
genomes and genes and noncoding regions of the mitochondrial genome may be used as a foundation for studying the evolutionary
histories of these genomes. Additionally, we identified seven highly variable genomic regions in the chloroplast genome upon
which a molecular phylogenetic investigation on an expanded set of samples from across the species’ geographic distribution can
be conducted to further define the taxonomic placements of P. tenuiflorum and P. floribundum.