化学试剂在非荧光药物标签中的应用综述

Pub Date : 2023-06-09 DOI:10.2174/1573412919666230609120450

B. Devi, K. Madhavi, Swathi Naraparaju, Rajeshwari Deverakonda

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引用次数: 0

摘要

荧光光谱法是一种高灵敏度、特异性和简便性的分析技术，非常经济。缺乏天然荧光的药物用试剂衍生，从而形成可以通过荧光光谱法容易检测的高荧光化合物。有不同的试剂如4-氯-7-硝基苯-2-氧杂-1,3-二唑（NBD-Cl）、N-甲基烟酰胺氯化物（NMNCl）、9-氟苯基甲基氯甲酸酯（FMOC-Cl）、丹酰氯（DNS-Cl），3-甲基-2-苯并噻唑啉酮腙盐酸盐（MBTH），1,2-萘醌-4-磺酸盐（NQS），1,4-苯醌，4-氟-7-硝基苯并呋喃赞（NBD-F），茚三酮，安息香，N-溴代琥珀酰亚胺（NBS），乙酰丙酮和六氯环己烷、邻苯二甲醛（OPA）和2-氰基乙酰胺用于荧光光谱法测定缺乏天然荧光的药物。本文着重介绍了这些试剂的原理、反应条件及其应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Use of Chemical Reagents in Non-Fluorescent Pharmaceutical Labels: A Comprehensive Review

Spectrofluorimetry is an analytical technique with high sensitivity, specificity, and simplicity, and is very economical. Drugs that lack native fluorescence are derivatized with reagents so that highly fluorescent compounds are formed that can be readily detected by spectrofluorimetry. There are different reagents such as 4-chloro-7-nitrobenz-2-oxa-1,3-diazole (NBD-Cl), N- methylnicotinamide chloride (NMNCl), 9-flurenylmethylchloroformate (FMOC-Cl), dansylchloride (DNS-Cl), 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH), 1,2-naphthoquinone-4-sulfonate (NQS), 1,4- benzoquinone, 4-fluro-7-nitrobenzofurazan (NBD-F), ninhydrin, benzoin, N- bromo succinimide (NBS), acetylacetone & HCHO, ortho-phthalaldehyde (OPA), and 2-cyanoacetamide used in spectrofluorimetric determination of the drugs that lack native fluorescence. The present review highlights these reagents with principle, reaction conditions, and their applications.

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