苦杏仁叶粉对肉鸡生长性能、肠黏膜活性、消化酶、吸收能力和免疫力的影响

Q3 Agricultural and Biological Sciences Journal of Applied Animal Nutrition Pub Date : 2023-02-14 DOI:10.3920/jaan2022.0006
B. M. Tokofai, B.M. Orounladji, K. Idoh, O. Oke, A. Agbonon
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引用次数: 1

摘要

肠道健康是多方面的,在很大程度上受饲养环境和饮食的影响。利用富含酚类和类黄酮的植物化学物质可以改善鸡的消化系统健康,提高鸡的生长性能。本试验旨在研究饲粮中添加苦杏仁叶粉(VALM)对肉仔鸡生长性能、消化酶活性、吸收功能、器官重量和免疫力的影响。选取240只1日龄雄性科布500肉鸡,随机分为4组,分别为不添加VALM的对照组和添加1、3和5 g/kg VALM的VA-1、VA-3和VA-5组。每个处理6个重复,每重复10只鸡。第42天,每个重复分离6只鸡并实施安乐死。收集空肠段(10 cm)食糜,进行消化酶分析。然后用冰冷的磷酸盐缓冲盐水冲洗剩余食糜,然后纵向打开空肠段(10 cm),通过刮拭收集粘膜。制备匀浆时,将肠黏膜样品用154 mmol/l的冰冷氯化钠溶液匀浆,4℃离心900 s。提取上清液,-20℃保存,测定免疫球蛋白、谷胱甘肽和d -木糖含量。在不同的处理中,添加VALM对器官的相对重量没有显著影响。3 g/kg VALM组淀粉酶和胰蛋白酶浓度显著升高(P<0.05)。饲粮3 G /kg VALM显著提高了免疫球蛋白A和肠道分泌免疫球蛋白G浓度(P<0.05)。这支持了饲料中添加3 g/kg VALM可以改善胃免疫状态和消化酶分泌的前提。
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Effect of Vernonia amygdalina leaf meal on growth performance, intestinal mucosa activity, digestive enzymes, absorption capacity, and immunity in broiler chickens
Gut health is multifaceted and is largely influenced by the rearing environment and the diet. The use of phytochemicals rich in phenolics and flavonoids can improve the digestive health of chickens and lead to better growth performance. The aim of this study was to examine the effects of dietary Vernonia amygdalina leaf meal (VALM) on growth performance, digestive enzyme activities, absorption function, organ weights and immunity of broilers. Two hundred and forty, one-day-old male Cobb 500 broiler chicks were randomly divided into four groups: an unsupplemented control and VA-1, VA-3 and VA-5 receiving VALM incorporation at concentrations of 1, 3 and 5 g/kg, respectively. Each treatment had six replicates of 10 chickens. On d 42, six chickens per replicate were isolated and euthanised. Digesta from the jejunal segments (10 cm) was collected for analysis of the digestive enzymes. The remaining digesta was then washed out with ice-cold phosphate-buffered saline before the jejunal segments (10 cm) were opened longitudinally to collect the mucosa by scraping. For the preparation of the homogenate, intestinal mucosa samples were homogenised with 154 mmol/l of ice-cold sodium chloride solution and centrifuged at 4 °C for 900 s. To determine immunoglobulins, glutathione and D-xylose, the supernatant was extracted and stored at -20 °C. Supplementation with VALM did not significantly influence the relative weights of organs in the different treatments. However, VALM at 3 g/kg caused a significant increase in amylase and trypsin concentration (P<0.05). Immunoglobulin A and intestinal secretory immunoglobulin G concentrations were significantly improved (P<0.05) in the birds fed 3 g/kg VALM. This supported the premise that 3 g/kg VALM in feed can improve gastric immunity status and digestive enzyme secretion.
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来源期刊
Journal of Applied Animal Nutrition
Journal of Applied Animal Nutrition Agricultural and Biological Sciences-Animal Science and Zoology
CiteScore
2.50
自引率
0.00%
发文量
11
审稿时长
10 weeks
期刊最新文献
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