植物基因改造:一项新兴技术

Krishnaben Desai, N. Modi
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引用次数: 0

摘要

转基因(GM)植物可以通过添加从相同植物物种或不同物种获得的特定DNA序列来创建。其目的是通过操纵基因表达来改变蛋白质特性,从而实现更高的产量、抗病虫害性、除草剂耐受性、抗体和其他药物分子的产生。抗虫植物可以减少病虫害造成的损害。除草剂草甘膦或耐草膦的转基因植物在对抗杂草方面取得了良好的效果。植物的特性,如金属吸收、运输、积累和有机污染物的解毒,可以通过基因操纵快速生长并从超积累物种中引入负责基因来增强。如果考虑到某些限制,如最终产品的质量、生物制药的提取和加工技术以及生物安全,转基因植物可以用于生产成本效益高且易于管理的药物、疫苗和生物制药。尽管有这些好处,但它对环境和人类健康的不利影响一直令人关切。主要限制包括转基因水平转移到其他物种,这可能编码特定的抗生素和除草剂抗性。这可能是通过肠道细菌将耐药性从食品传播到整个人类。为了解决这一问题,需要采取几种方法,在田间生长之前,远离或消除转化植物中的标记基因。许多科学家已经提出了产生无标记转基因植物的策略,为我们提供安全可靠的转基因技术。
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Genetic Modification of Plants: An Emerging Technology
Genetically modified (GM) plants can be created by adding specific DNA sequences obtained from the same plant species or different species. Which aims to achieve higher yields, disease and pest resistance, herbicide tolerance, production of antibodies, and other pharmaceutical molecules by manipulating gene expression to alter the protein properties. Insect resistant plants reduce the damage caused by pests and diseases. Herbicide glyphosate or glufosinate-tolerant GM plants gave promising results in combating weed. The properties of plants such as metal uptake, transport, accumulation, and detoxification of organic pollutants can be enhanced by genetically manipulating the fast-grown and introducing the responsible gene from the hyper accumulative species. GM plants can be used to produce cost-efficient and manageable drugs, vaccine, and biopharmaceuticals, if certain limitations are to be considered such as quality of final products, techniques for extraction and processing of biopharmaceuticals, and biosafety. Despite all these benefits, its adverse effects on the environment and human health have always been a matter of concern. The main limitation includes a horizontal transfer of the transgene to other species which may code for the specific antibiotic and herbicide resistance. It might be the possible transmission of resistance from the food products to the whole human population via intestinal bacteria. To address this several methods, need to be adopted to either keep away or eliminate marker genes from the transformed plants before growing in the field. Many scientists have come up with strategies to generate marker-free transgenic plants to give us safe and reliable GM technology.
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