{"title":"trim28（ser473）磷酸化对肝细胞癌细胞DNA损伤生存能力的影响","authors":"Yongliang Wang, S. Zhai, Bailin Wang, Wei Yuan, Pengzhen Wang, Xu Zhang, Weilu Jia, Hui Yuan","doi":"10.3760/CMA.J.ISSN.1001-9030.2019.12.018","DOIUrl":null,"url":null,"abstract":"Objective \nTo observe effect of Trim28 (ser473) phosphorylation on viability of hepatocellular carcinoma (HCC) cells during DNA damage. \n \n \nMethods \nTo divide Hepg2 cells into 2 groups as SB203580+ UV group and UV group. Hepg2 cells were modeled for DNA damage by UV (Ultraviolet) irradiation. SB203580+ UV group was treated with Trim28 phosphorylation inhibitor SB203580 after UV irradiation. Expression of Trim28 and phosphorylation-Trim28 ser473 (P-Trim28 ser473)were detected by westernblot experiment. The viability of Hepg2 cells were observed by methyl thiazol tetrazolium (MTT) experiment. \n \n \nResults \nCompared with UV group (0.94±0.13), the expression value of Trim28 in SB203580+ UV group had no significant difference (t=-0.190, P>0.05). Value of phosphorylation-Trim28 in SB203580+ UV group (0.32±0.04) was obviously lower than UV group (0.72±0.05), the difference had statistical meaning (t=8.500, P<0.05); The 24, 48 and 72 hours after UV irradiation, the cell survive rate of UV group [(95±7)%, (62±10)%, (35±6)%] is higher than SB203580+ UV group [(85±7)%, (37±4)%, (20±3)%]. The difference has statistical meaning (t=-2.326, -5.212, -4.577, P<0.05). \n \n \nConclusion \nThe viability of HCC cells during DNA damaged can be reduced by inhibition for Trim28 phosphorylation. \n \n \nKey words: \nTrim28; Phosphorylation; Cell survival","PeriodicalId":10065,"journal":{"name":"中华实验外科杂志","volume":"36 1","pages":"2187-2189"},"PeriodicalIF":0.0000,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of trim28 (ser473) phosphorylation on viability of hepatocellular carcinoma cells during DNA damage\",\"authors\":\"Yongliang Wang, S. Zhai, Bailin Wang, Wei Yuan, Pengzhen Wang, Xu Zhang, Weilu Jia, Hui Yuan\",\"doi\":\"10.3760/CMA.J.ISSN.1001-9030.2019.12.018\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective \\nTo observe effect of Trim28 (ser473) phosphorylation on viability of hepatocellular carcinoma (HCC) cells during DNA damage. \\n \\n \\nMethods \\nTo divide Hepg2 cells into 2 groups as SB203580+ UV group and UV group. Hepg2 cells were modeled for DNA damage by UV (Ultraviolet) irradiation. SB203580+ UV group was treated with Trim28 phosphorylation inhibitor SB203580 after UV irradiation. Expression of Trim28 and phosphorylation-Trim28 ser473 (P-Trim28 ser473)were detected by westernblot experiment. The viability of Hepg2 cells were observed by methyl thiazol tetrazolium (MTT) experiment. \\n \\n \\nResults \\nCompared with UV group (0.94±0.13), the expression value of Trim28 in SB203580+ UV group had no significant difference (t=-0.190, P>0.05). Value of phosphorylation-Trim28 in SB203580+ UV group (0.32±0.04) was obviously lower than UV group (0.72±0.05), the difference had statistical meaning (t=8.500, P<0.05); The 24, 48 and 72 hours after UV irradiation, the cell survive rate of UV group [(95±7)%, (62±10)%, (35±6)%] is higher than SB203580+ UV group [(85±7)%, (37±4)%, (20±3)%]. The difference has statistical meaning (t=-2.326, -5.212, -4.577, P<0.05). \\n \\n \\nConclusion \\nThe viability of HCC cells during DNA damaged can be reduced by inhibition for Trim28 phosphorylation. \\n \\n \\nKey words: \\nTrim28; Phosphorylation; Cell survival\",\"PeriodicalId\":10065,\"journal\":{\"name\":\"中华实验外科杂志\",\"volume\":\"36 1\",\"pages\":\"2187-2189\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-12-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中华实验外科杂志\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2019.12.018\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中华实验外科杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2019.12.018","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Effect of trim28 (ser473) phosphorylation on viability of hepatocellular carcinoma cells during DNA damage
Objective
To observe effect of Trim28 (ser473) phosphorylation on viability of hepatocellular carcinoma (HCC) cells during DNA damage.
Methods
To divide Hepg2 cells into 2 groups as SB203580+ UV group and UV group. Hepg2 cells were modeled for DNA damage by UV (Ultraviolet) irradiation. SB203580+ UV group was treated with Trim28 phosphorylation inhibitor SB203580 after UV irradiation. Expression of Trim28 and phosphorylation-Trim28 ser473 (P-Trim28 ser473)were detected by westernblot experiment. The viability of Hepg2 cells were observed by methyl thiazol tetrazolium (MTT) experiment.
Results
Compared with UV group (0.94±0.13), the expression value of Trim28 in SB203580+ UV group had no significant difference (t=-0.190, P>0.05). Value of phosphorylation-Trim28 in SB203580+ UV group (0.32±0.04) was obviously lower than UV group (0.72±0.05), the difference had statistical meaning (t=8.500, P<0.05); The 24, 48 and 72 hours after UV irradiation, the cell survive rate of UV group [(95±7)%, (62±10)%, (35±6)%] is higher than SB203580+ UV group [(85±7)%, (37±4)%, (20±3)%]. The difference has statistical meaning (t=-2.326, -5.212, -4.577, P<0.05).
Conclusion
The viability of HCC cells during DNA damaged can be reduced by inhibition for Trim28 phosphorylation.
Key words:
Trim28; Phosphorylation; Cell survival
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