T. Kavetskyy, N. Stasyuk, O. Smutok, O. Demkiv, Y. Kukhazh, N. Hoivanovych, V. Boev, V. Ilcheva, T. Petkova, M. Gonchar
{"title":"用酶固定金纳米颗粒偶联脲脲聚合物作为宿主基质改进安培漆酶生物传感器","authors":"T. Kavetskyy, N. Stasyuk, O. Smutok, O. Demkiv, Y. Kukhazh, N. Hoivanovych, V. Boev, V. Ilcheva, T. Petkova, M. Gonchar","doi":"10.1007/s13404-019-00255-z","DOIUrl":null,"url":null,"abstract":"<p>Synthesis of gold nanoparticles (Au-NPs) was performed using method of an inorganic chemical reduction. The synthesized Au-NPs showed an intensive surface plasmon resonance band at 525?nm that is typical for corresponding Au-NPs reported in literature. The structural and morphology characterizations of the obtained Au-NPs were carried out using SEM, AFM, TEM, and X-ray spectral analysis. The possibility of usage of Au-NPs as a carrier for covalent immobilization of commercial laccase and the combination of the obtained bio-Au-NPs with ureasil polymers as host matrixes for formation of bio-nanocomposite films was studied. The prospect of using the obtained bio-nanocomposite films in biosensor technologies in order to improve the bioanalytical characteristics of sensory elements has been proved.</p>","PeriodicalId":581,"journal":{"name":"Gold Bulletin","volume":"52 2","pages":"79 - 85"},"PeriodicalIF":2.1000,"publicationDate":"2019-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s13404-019-00255-z","citationCount":"16","resultStr":"{\"title\":\"Improvement of amperometric laccase biosensor using enzyme-immobilized gold nanoparticles coupling with ureasil polymer as a host matrix\",\"authors\":\"T. Kavetskyy, N. Stasyuk, O. Smutok, O. Demkiv, Y. Kukhazh, N. Hoivanovych, V. Boev, V. Ilcheva, T. Petkova, M. Gonchar\",\"doi\":\"10.1007/s13404-019-00255-z\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Synthesis of gold nanoparticles (Au-NPs) was performed using method of an inorganic chemical reduction. The synthesized Au-NPs showed an intensive surface plasmon resonance band at 525?nm that is typical for corresponding Au-NPs reported in literature. The structural and morphology characterizations of the obtained Au-NPs were carried out using SEM, AFM, TEM, and X-ray spectral analysis. The possibility of usage of Au-NPs as a carrier for covalent immobilization of commercial laccase and the combination of the obtained bio-Au-NPs with ureasil polymers as host matrixes for formation of bio-nanocomposite films was studied. The prospect of using the obtained bio-nanocomposite films in biosensor technologies in order to improve the bioanalytical characteristics of sensory elements has been proved.</p>\",\"PeriodicalId\":581,\"journal\":{\"name\":\"Gold Bulletin\",\"volume\":\"52 2\",\"pages\":\"79 - 85\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2019-05-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1007/s13404-019-00255-z\",\"citationCount\":\"16\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Gold Bulletin\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s13404-019-00255-z\",\"RegionNum\":4,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, INORGANIC & NUCLEAR\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gold Bulletin","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s13404-019-00255-z","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, INORGANIC & NUCLEAR","Score":null,"Total":0}
Improvement of amperometric laccase biosensor using enzyme-immobilized gold nanoparticles coupling with ureasil polymer as a host matrix
Synthesis of gold nanoparticles (Au-NPs) was performed using method of an inorganic chemical reduction. The synthesized Au-NPs showed an intensive surface plasmon resonance band at 525?nm that is typical for corresponding Au-NPs reported in literature. The structural and morphology characterizations of the obtained Au-NPs were carried out using SEM, AFM, TEM, and X-ray spectral analysis. The possibility of usage of Au-NPs as a carrier for covalent immobilization of commercial laccase and the combination of the obtained bio-Au-NPs with ureasil polymers as host matrixes for formation of bio-nanocomposite films was studied. The prospect of using the obtained bio-nanocomposite films in biosensor technologies in order to improve the bioanalytical characteristics of sensory elements has been proved.
期刊介绍：
Gold Bulletin is the premier international peer reviewed journal on the latest science, technology and applications of gold. It includes papers on the latest research advances, state-of-the-art reviews, conference reports, book reviews and highlights of patents and scientific literature. Gold Bulletin does not publish manuscripts covering the snthesis of Gold nanoparticles in the presence of plant extracts or other nature-derived extracts. Gold Bulletin has been published over 40 years as a multidisciplinary journal read by chemists, physicists, engineers, metallurgists, materials scientists, biotechnologists, surface scientists, and nanotechnologists amongst others, both within industry and academia. Gold Bulletin is published in Association with the World Gold Council.
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