{"title":"大鼠脑缺血模型","authors":"Zeki Serdar Ataizi","doi":"10.37212/jcnos.610115","DOIUrl":null,"url":null,"abstract":"Stroke is the second cause of death worldwide. Stroke induces cerebral ischemia. The cerebral ischemia is a neurodegenerative disease that causes disability and mortality. An accumulating body of evidence indicates that abnormalities of Ca2+ homeostasis are caused by excessive levels of free oxygen radicals in rats with cerebral ischemia. Occlusion of middle cerebral artery in human induces cerebral ischemic stroke. In experimental animals, best model of induction of cerebral ischemic stroke is occlusion of middle cerebral artery for 30 min (Canazza et al. 2014). In cerebral ischemia stoke model, right or left middle cerebral artery is exposed through a ventral midline incision in the neck and it is loosely encircled with sutures for further occlusion. Following a midline incision, the skull is craniectomized to expose the right or left common carotid artery. A 3-0 suture is positioned so that it encircled the middle cerebral artery for further occlusion. Cerebral ischemic surgery is performed through occlusion of the right or left middle cerebral artery for 30 min (Akpinar et al. 2016). In addition to the best model, there are also other models of cerebral stroke in rodents such as the intra-luminal suture, thromboembolic, the coagulation or ligation, the endothelin-1, and the distal artery compression models (Canazza et al. 2014). In the presentation, a selection of the principal models is described and the model was compared with the other models.","PeriodicalId":37782,"journal":{"name":"Journal of Cellular Neuroscience and Oxidative Stress","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2018-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Cerebral ischemia models in rats\",\"authors\":\"Zeki Serdar Ataizi\",\"doi\":\"10.37212/jcnos.610115\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Stroke is the second cause of death worldwide. Stroke induces cerebral ischemia. The cerebral ischemia is a neurodegenerative disease that causes disability and mortality. An accumulating body of evidence indicates that abnormalities of Ca2+ homeostasis are caused by excessive levels of free oxygen radicals in rats with cerebral ischemia. Occlusion of middle cerebral artery in human induces cerebral ischemic stroke. In experimental animals, best model of induction of cerebral ischemic stroke is occlusion of middle cerebral artery for 30 min (Canazza et al. 2014). In cerebral ischemia stoke model, right or left middle cerebral artery is exposed through a ventral midline incision in the neck and it is loosely encircled with sutures for further occlusion. Following a midline incision, the skull is craniectomized to expose the right or left common carotid artery. A 3-0 suture is positioned so that it encircled the middle cerebral artery for further occlusion. Cerebral ischemic surgery is performed through occlusion of the right or left middle cerebral artery for 30 min (Akpinar et al. 2016). In addition to the best model, there are also other models of cerebral stroke in rodents such as the intra-luminal suture, thromboembolic, the coagulation or ligation, the endothelin-1, and the distal artery compression models (Canazza et al. 2014). In the presentation, a selection of the principal models is described and the model was compared with the other models.\",\"PeriodicalId\":37782,\"journal\":{\"name\":\"Journal of Cellular Neuroscience and Oxidative Stress\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-08-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Cellular Neuroscience and Oxidative Stress\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.37212/jcnos.610115\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Cellular Neuroscience and Oxidative Stress","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.37212/jcnos.610115","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
Stroke is the second cause of death worldwide. Stroke induces cerebral ischemia. The cerebral ischemia is a neurodegenerative disease that causes disability and mortality. An accumulating body of evidence indicates that abnormalities of Ca2+ homeostasis are caused by excessive levels of free oxygen radicals in rats with cerebral ischemia. Occlusion of middle cerebral artery in human induces cerebral ischemic stroke. In experimental animals, best model of induction of cerebral ischemic stroke is occlusion of middle cerebral artery for 30 min (Canazza et al. 2014). In cerebral ischemia stoke model, right or left middle cerebral artery is exposed through a ventral midline incision in the neck and it is loosely encircled with sutures for further occlusion. Following a midline incision, the skull is craniectomized to expose the right or left common carotid artery. A 3-0 suture is positioned so that it encircled the middle cerebral artery for further occlusion. Cerebral ischemic surgery is performed through occlusion of the right or left middle cerebral artery for 30 min (Akpinar et al. 2016). In addition to the best model, there are also other models of cerebral stroke in rodents such as the intra-luminal suture, thromboembolic, the coagulation or ligation, the endothelin-1, and the distal artery compression models (Canazza et al. 2014). In the presentation, a selection of the principal models is described and the model was compared with the other models.
期刊介绍:
Journal of Cellular Neuroscience and Oxidative Stress isan online journal that publishes original research articles, reviews and short reviews on themolecular basisofbiophysical,physiological and pharmacological processes thatregulate cellular function, and the control or alteration of these processesby theaction of receptors, neurotransmitters, second messengers, cation, anions,drugsor disease. Areas of particular interest are four topics. They are; 1. Ion Channels (Na+-K+Channels, Cl– channels, Ca2+channels, ADP-Ribose and metabolism of NAD+,Patch-Clamp applications) 2. Oxidative Stress (Antioxidant vitamins, antioxidant enzymes, metabolism of nitric oxide, oxidative stress, biophysics, biochemistry and physiology of free oxygen radicals) 3. Interaction Between Oxidative Stress and Ion Channels in Neuroscience (Effects of the oxidative stress on the activation of the voltage sensitive cation channels, effect of ADP-Ribose and NAD+ on activation of the cation channels which are sensitive to voltage, effect of the oxidative stress on activation of the TRP channels in neurodegenerative diseases such Parkinson’s and Alzheimer’s diseases) 4. Gene and Oxidative Stress (Gene abnormalities. Interaction between gene and free radicals. Gene anomalies and iron. Role of radiation and cancer on gene polymorphism)