哈萨克斯坦东南部冰草的遗传分析和分子特征

IF 1.6 Q3 PLANT SCIENCES Sabrao Journal of Breeding and Genetics Pub Date : 2023-08-31 DOI:10.54910/sabrao2023.55.4.10
B. Ainebekova
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引用次数: 1

摘要

在哈萨克斯坦共和国的环境条件下,冰草(Agropyron cristatum L.Gaertn.)多样性的组装、遗传分析和保存是改善上述作物的重要任务。本研究根据遗传学原理,从全球气候变化的角度评估和分离了各种麦草物种及其种群,以培育和开发有前景的品种,从而改善哈萨克斯坦东南部退化的牧场状况。在随后的调查中,收集了来自哈萨克斯坦不同地区的冰草地方品种,以及已经保存在基因库中的材料,并在哈萨克斯坦东南部的环境条件下进行了评估。在基于性能的筛选中,使用ISSRs聚合酶链反应标记对鉴定的20个麦草品种进行了进一步的基因分型。相关研究有助于了解冰草属植物的遗传结构和分子特征。利用7个ISSR引物研究了从不同地点采集的5个冰草属植物的亲缘关系和遗传多态性。ISSR引物对测定冰草的遗传多样性和种间相似性具有重要的参考价值。此外,强烈建议对国家生物技术中心数据库中的冰草种群的DNA测序进行深入研究。
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GENETIC ANALYSIS AND MOLECULAR CHARACTERIZATION OF THE WHEATGRASS (AGROPYRON CRISTATUM L. GAERTN.) IN SOUTH-EAST KAZAKHSTAN
Assembly, genetic analysis, and preservation of wheatgrass (Agropyron cristatum L. Gaertn.) diversity is an important task in improving the said crop under the environmental conditions of the Republic of Kazakhstan. The presented study evaluated and isolated various wheatgrass species and their populations for breeding and developing promising cultivars based on the genetic principles to improve the degraded pasture situation in South-East Kazakhstan from the perspective of global climate change. For the succeeding investigations, collection of the wheatgrass landraces from various regions of Kazakhstan ensued, as well as from accessions already conserved in the gene pool, and gained evaluation under the environmental conditions of South-East Kazakhstan. In performance-based screening, 20 wheatgrass cultivars identified underwent further genotyping by using inter-simple sequence repeat (ISSRs) polymerase chain reaction markers. The pertinent study significantly contributes to understanding the Agropyron species’ genetic structure and molecular characteristics. Using seven ISSR primers helped study the kinship and genetic polymorphism of the five Agropyron species collected from diverse locations. The ISSR primers were extremely informative for determining the Agropyron’s genetic diversity and interspecific similarity. Moreover, they come highly recommended for an in-depth study comparing the DNA sequencing of the Agropyron populations available in the National Center for Biotechnology’s database.
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来源期刊
Sabrao Journal of Breeding and Genetics
Sabrao Journal of Breeding and Genetics 农林科学-奶制品与动物科学
CiteScore
1.90
自引率
50.00%
发文量
63
期刊介绍: The SABRAO Journal of Breeding and Genetics is an international journal of plant breeding and genetics research and was first published in 1969. It is the official publication of the Society for the Advancement of Breeding Research in Asia and Oceania (SABRAO). Its objectives are to: promote the international exchange of research information on plant breeding and genetics, by describing new research findings, or ideas of a basic or practical nature; and be a medium for the exchange of ideas and news regarding members of the Society. The Journal gives priority to articles that are of direct relevance to plant breeders and with emphasis on the Asian region. Invited for publication are research articles, short communications, methods, reviews, commentaries, and opinion articles. Scientific contributions are refereed and edited to international standards. The journal publishes articles for SABRAO members mainly. The Journal preferred strongly that at least one author should be a current member of the Society. Non-members may also publish in the journal.
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