Sung-Jong Cho, Jeonghee Lee, I. Yang, Jinsoon Park
{"title":"韩国南部海岸日本铁的分子鉴定","authors":"Sung-Jong Cho, Jeonghee Lee, I. Yang, Jinsoon Park","doi":"10.1080/13235818.2023.2217531","DOIUrl":null,"url":null,"abstract":"ABSTRACT This study represents the first molecular analysis of Japeuthria ferrea specimens from Korea, focusing on COI and H3 gene regions for species identification and phylogenetic analysis. A total of 13 samples was collected from three different locations along the south coast of Korea, and their sequences were registered in the NCBI GenBank. The COI and H3 gene regions were successfully amplified and bidirectionally sequenced, providing a valuable reference for future DNA barcoding studies. Phylogenetic analysis based on both COI and H3 sequences demonstrated that the Korean and Japanese J. ferrea populations lacked clear regional differentiation. Despite the limitations in differentiating the regional characteristics of J. ferrea, the study confirms the applicability of COI and H3 genes for accurate species identification. The findings emphasise the importance of employing multiple molecular markers for comprehensive DNA analysis to improve species identification accuracy and to minimise misidentification. Further investigation involving expanded sampling and additional DNA regions is required to elucidate the phylogenetic relationships and distribution of J. ferrea.","PeriodicalId":18857,"journal":{"name":"Molluscan Research","volume":"43 1","pages":"130 - 137"},"PeriodicalIF":0.8000,"publicationDate":"2023-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular identification of Japeuthria ferrea from the south coast of Korea\",\"authors\":\"Sung-Jong Cho, Jeonghee Lee, I. Yang, Jinsoon Park\",\"doi\":\"10.1080/13235818.2023.2217531\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"ABSTRACT This study represents the first molecular analysis of Japeuthria ferrea specimens from Korea, focusing on COI and H3 gene regions for species identification and phylogenetic analysis. A total of 13 samples was collected from three different locations along the south coast of Korea, and their sequences were registered in the NCBI GenBank. The COI and H3 gene regions were successfully amplified and bidirectionally sequenced, providing a valuable reference for future DNA barcoding studies. Phylogenetic analysis based on both COI and H3 sequences demonstrated that the Korean and Japanese J. ferrea populations lacked clear regional differentiation. Despite the limitations in differentiating the regional characteristics of J. ferrea, the study confirms the applicability of COI and H3 genes for accurate species identification. The findings emphasise the importance of employing multiple molecular markers for comprehensive DNA analysis to improve species identification accuracy and to minimise misidentification. Further investigation involving expanded sampling and additional DNA regions is required to elucidate the phylogenetic relationships and distribution of J. ferrea.\",\"PeriodicalId\":18857,\"journal\":{\"name\":\"Molluscan Research\",\"volume\":\"43 1\",\"pages\":\"130 - 137\"},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2023-04-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molluscan Research\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1080/13235818.2023.2217531\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ZOOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molluscan Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/13235818.2023.2217531","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ZOOLOGY","Score":null,"Total":0}
Molecular identification of Japeuthria ferrea from the south coast of Korea
ABSTRACT This study represents the first molecular analysis of Japeuthria ferrea specimens from Korea, focusing on COI and H3 gene regions for species identification and phylogenetic analysis. A total of 13 samples was collected from three different locations along the south coast of Korea, and their sequences were registered in the NCBI GenBank. The COI and H3 gene regions were successfully amplified and bidirectionally sequenced, providing a valuable reference for future DNA barcoding studies. Phylogenetic analysis based on both COI and H3 sequences demonstrated that the Korean and Japanese J. ferrea populations lacked clear regional differentiation. Despite the limitations in differentiating the regional characteristics of J. ferrea, the study confirms the applicability of COI and H3 genes for accurate species identification. The findings emphasise the importance of employing multiple molecular markers for comprehensive DNA analysis to improve species identification accuracy and to minimise misidentification. Further investigation involving expanded sampling and additional DNA regions is required to elucidate the phylogenetic relationships and distribution of J. ferrea.
期刊介绍:
Molluscan Research is an international journal for the publication of authoritative papers and review articles on all aspects of molluscan research, including biology, systematics, morphology, physiology, ecology, conservation, biogeography, genetics, molecular biology and palaeontology.
While the scope of the journal is worldwide, there is emphasis on studies relating to Australasia and the Indo-west Pacific, including East and South East Asia. The journal’s scope includes revisionary papers, monographs, reviews, theoretical papers and briefer communications. Monographic studies of up to 73 printed pages may also be considered.
The journal has been published since 1957 (as the Journal of the Malacological Society of Australia until 1993). It is free to members of the Malacological Society of Australasia and the Society for the Study of Molluscan Diversity.