纳米颗粒脂质体:细菌感染的新策略

IF 0.3 Q3 MEDICINE, GENERAL & INTERNAL Klimik Journal Pub Date : 2021-07-09 DOI:10.36519/KD.2021.3633
Perihan Erkan-Alkan, M. Güneş, C. Özakın, A. Sabancı
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引用次数: 1

摘要

目的:本研究使用专利申请号为TR201804452A2的纳米颗粒脂质体分子,其最小抑制剂浓度(MIC)为1562 ppm。根据ASTM F 1980标准,已经确定纳米颗粒脂质体溶液在37天和55℃的条件下保持一年的稳定性可以保持其有效性。我们的研究旨在证明新开发的解决方案在很长一段时间内保持其有效性。方法:CLSI M07-A10(好氧生长细菌稀释药敏试验方法)。采用不同于标准臭氧化机制的技术开发了纳米颗粒脂质体溶液的标准试验方法,并通过改变溶液的接触时间和MIC值进行了抗菌试验,并确定了其对时间的影响。对于纳米颗粒脂质体溶液的稳定性测试,根据ASTM F 1980标准,在55℃下保存37天,以获得一年的稳定性。结果:纳米颗粒臭氧溶液CLSI M07-A10标准试验方法对金黄色葡萄球菌(ATCC 25923)和大肠杆菌(ATCC 25922)菌株的MIC测定值为1.562 ppm。对于金黄色葡萄球菌(ATCC 25923),在第一个小时结束时,测定其活性始于2000和1750 ppm纳米颗粒脂质体溶液浓度。对于大肠杆菌(ATCC 25922),测定其活性在2000 ppm纳米粒子臭氧溶液浓度下的第10分钟开始。根据ASTM F 1980的有效性标准,该解决方案在一年后仍然有效。结论:纳米颗粒脂质体溶液作为一种新产品,在很长一段时间内并没有失去其稳定性和有效性,这与已知的情况相反。虽然气态臭氧的半衰期短至20分钟,但在纳米颗粒脂质体溶液中的稳定性已被确定为至少一年。由于纳米颗粒脂质体溶液是一种天然的缓释产品,因此人们认为,它可以在鼻、喉、眼和耳等区域的粘膜上形成屏障,并以适当剂量配制溶液,从而防止细菌沉淀。关键词:纳米颗粒脂质体,臭氧,抗菌效率,金黄色葡萄球菌(ATCC 25923),大肠杆菌(ATCC 25922)
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Nanoparticle Liposomes: a New Strategy in Bacterial Infections
Objective: In our study, a nanoparticle liposome molecule with patent application number TR201804452A2 was used, and the Minimum Inhibitor Concentration (MIC) was found to be 1562 ppm. According to the ASTM F 1980 standard, it has been determined that the nanoparticle liposome solution kept at 37 days and 55 oC in return for one-year stability preserves its effectiveness. Our study aimed to show that the newly developed solution maintains its effectiveness for a long time. Methods: CLSI M07-A10 (Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically. Tenth ed. Approved Standard) standard test method of the nanoparticle liposome solution developed with a technique different from the standard ozonation mechanisms, and antibacterial tests were performed by modifying the contact time and the MIC value of the solution, and its effect on time has been determined. For the stability test of the nanoparticle liposome solution, it was kept at 55 oC for 37 days in return for one-year stability according to the ASTM F 1980 standard. Results: MIC of nanoparticular ozone solution CLSI M07-A10 standard test method for S. aureus (ATCC 25923) and E. coli (ATCC 25922) strains by modifying contact time It was determined as 1.562 ppm. For S. aureus (ATCC 25923), at the end of the first hour, it was determined that the activity started at 2000 and 1750 ppm nanoparticle liposome solution concentration. For E. coli (ATCC 25922) it was determined that the activity started at the 10th minute at 2000 ppm nanoparticular ozone solution concentration. The solution was still effective at the end of one year according to the ASTM F 1980 standard in terms of effectiveness. Conclusions: As a result, the nanoparticle liposome solution, a new product, does not lose its stability and effectiveness for a long time, contrary to what is known. Although the half-life of gaseous ozone is as short as 20 minutes, the stability in the nanoparticle liposome solution has been determined as at least one year. Since nanoparticle liposome solution is a natural and slow-release product, it is thought that it can create a barrier in mucosal membranes in regions such as the nose, throat, eye and ear with solutions to be prepared in appropriate doses thus preventing bacteria from settling. Keywords: Nanoparticle liposomes, ozone, antibacterial efficiency, Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922)
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Klimik Journal
Klimik Journal MEDICINE, GENERAL & INTERNAL-
CiteScore
0.60
自引率
33.30%
发文量
39
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