{"title":"两种剂量eCG用于羊驼胚胎获取的比较","authors":"Cesar Olaguivel, J. Ruíz, Pedro U. Coila","doi":"10.18548/aspe/0009.23","DOIUrl":null,"url":null,"abstract":"The study was developed at the Pampa del Arco Experimental Center of the National University of San Cristóbal de Huamanga, the objective was to evaluate doses of 800 IU (T1) and doses of 1000 IU (T2) of eCG to obtain embryos. Eight adult female llamas were used, distributed four in each treatment (T1 and T2), with a body condition of 2.5, these were evaluated by ultrasound with a 7.5 MHz rectal linear transducer. Hormonal treatments began when the animals had a follicle> 7 mm. The application of eCG was 72 hours after the induction of ovulation of the dominant follicle with GnRH, on day 7 PGF2α was applied, natural mounting was performed plus GnRH (1ml), on day 15 the collection and evaluation of embryos was performed. The results obtained were the pre-ovarian stimulation follicular diameter of 8.3 mm and 9.1 mm for T1 and T2 respectively, the number of pre-ovulatory follicles was 6.33 and 5.50 for T1 and T2 respectively, the diameter of follicles pre-ovulatory was 11.2mm and 10.6 mm for T1 and T2 respectively, average of embryos recovered per donor was 4.66 ± 0.81 and 3.50 ± 0.54 embryos for T1 and T2 respectively, with a significant difference (p <0.05) and the quality of embryos had 35.7% and 38.1% of embryos of excellent quality, 28.6% and 33.3% of good quality, 10.7% and 9 , 5% of regular quality, 10.7% and 0% of poor quality, for T1 and T2 respectively and 14.3% and 19.0% of non-transferable embryos for T1 and T2 respectively, with no statistical difference (p≥0, 05). It is concluded that the number of embryos recovered with a dose of 800 IU of eCG is higher compared to the dose of 1000 IU of eCG (p <0.05) and that there is no association between the applied dose of eCG and the quality of the llama embryos (p≥0.05)","PeriodicalId":36778,"journal":{"name":"Spermova","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparison of two doses of eCG for obtaining embryos in llama\",\"authors\":\"Cesar Olaguivel, J. Ruíz, Pedro U. Coila\",\"doi\":\"10.18548/aspe/0009.23\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The study was developed at the Pampa del Arco Experimental Center of the National University of San Cristóbal de Huamanga, the objective was to evaluate doses of 800 IU (T1) and doses of 1000 IU (T2) of eCG to obtain embryos. Eight adult female llamas were used, distributed four in each treatment (T1 and T2), with a body condition of 2.5, these were evaluated by ultrasound with a 7.5 MHz rectal linear transducer. Hormonal treatments began when the animals had a follicle> 7 mm. The application of eCG was 72 hours after the induction of ovulation of the dominant follicle with GnRH, on day 7 PGF2α was applied, natural mounting was performed plus GnRH (1ml), on day 15 the collection and evaluation of embryos was performed. The results obtained were the pre-ovarian stimulation follicular diameter of 8.3 mm and 9.1 mm for T1 and T2 respectively, the number of pre-ovulatory follicles was 6.33 and 5.50 for T1 and T2 respectively, the diameter of follicles pre-ovulatory was 11.2mm and 10.6 mm for T1 and T2 respectively, average of embryos recovered per donor was 4.66 ± 0.81 and 3.50 ± 0.54 embryos for T1 and T2 respectively, with a significant difference (p <0.05) and the quality of embryos had 35.7% and 38.1% of embryos of excellent quality, 28.6% and 33.3% of good quality, 10.7% and 9 , 5% of regular quality, 10.7% and 0% of poor quality, for T1 and T2 respectively and 14.3% and 19.0% of non-transferable embryos for T1 and T2 respectively, with no statistical difference (p≥0, 05). It is concluded that the number of embryos recovered with a dose of 800 IU of eCG is higher compared to the dose of 1000 IU of eCG (p <0.05) and that there is no association between the applied dose of eCG and the quality of the llama embryos (p≥0.05)\",\"PeriodicalId\":36778,\"journal\":{\"name\":\"Spermova\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-12-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Spermova\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18548/aspe/0009.23\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Veterinary\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Spermova","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18548/aspe/0009.23","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Veterinary","Score":null,"Total":0}
引用次数: 0
摘要
这项研究是在San国立大学Cristóbal de Huamanga Pampa del Arco实验中心开展的,目的是评估800 IU (T1)剂量和1000 IU (T2)剂量的eCG获得胚胎的效果。选用成年雌性大羊驼8只,体况2.5,T1和T2各4只,采用7.5 MHz直肠线性换向器进行超声检测。激素治疗开始时,动物的卵泡有一个70毫米。在GnRH诱导优势卵泡排卵后72h应用eCG,第7天应用PGF2α,加GnRH (1ml)自然贴装,第15天收集和评估胚胎。结果:T1和T2促卵巢前卵泡直径分别为8.3 mm和9.1 mm, T1和T2促排卵前卵泡数分别为6.33和5.50个,T1和T2促排卵前卵泡直径分别为11.2mm和10.6 mm, T1和T2平均每个供体回收的胚胎分别为4.66±0.81和3.50±0.54个。,差异有统计学意义(p <0.05), T1和T2的优良率分别为35.7%和38.1%,优良率分别为28.6%和33.3%,正常率分别为10.7%和9.5%,差质率分别为10.7%和0%,T1和T2的不可移植率分别为14.3%和19.0%,差异无统计学意义(p≥0.05)。综上所述,800 IU剂量下恢复的胚胎数量高于1000 IU剂量(p <0.05), eCG剂量与羊驼胚胎质量无相关性(p≥0.05)。
Comparison of two doses of eCG for obtaining embryos in llama
The study was developed at the Pampa del Arco Experimental Center of the National University of San Cristóbal de Huamanga, the objective was to evaluate doses of 800 IU (T1) and doses of 1000 IU (T2) of eCG to obtain embryos. Eight adult female llamas were used, distributed four in each treatment (T1 and T2), with a body condition of 2.5, these were evaluated by ultrasound with a 7.5 MHz rectal linear transducer. Hormonal treatments began when the animals had a follicle> 7 mm. The application of eCG was 72 hours after the induction of ovulation of the dominant follicle with GnRH, on day 7 PGF2α was applied, natural mounting was performed plus GnRH (1ml), on day 15 the collection and evaluation of embryos was performed. The results obtained were the pre-ovarian stimulation follicular diameter of 8.3 mm and 9.1 mm for T1 and T2 respectively, the number of pre-ovulatory follicles was 6.33 and 5.50 for T1 and T2 respectively, the diameter of follicles pre-ovulatory was 11.2mm and 10.6 mm for T1 and T2 respectively, average of embryos recovered per donor was 4.66 ± 0.81 and 3.50 ± 0.54 embryos for T1 and T2 respectively, with a significant difference (p <0.05) and the quality of embryos had 35.7% and 38.1% of embryos of excellent quality, 28.6% and 33.3% of good quality, 10.7% and 9 , 5% of regular quality, 10.7% and 0% of poor quality, for T1 and T2 respectively and 14.3% and 19.0% of non-transferable embryos for T1 and T2 respectively, with no statistical difference (p≥0, 05). It is concluded that the number of embryos recovered with a dose of 800 IU of eCG is higher compared to the dose of 1000 IU of eCG (p <0.05) and that there is no association between the applied dose of eCG and the quality of the llama embryos (p≥0.05)