用于宏基因组学的鼻拭子样本微生物DNA富集技术评价

B. Mackenzie, D. Waite, K. Biswas, R. Douglas, Michael W Taylor
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引用次数: 6

摘要

方法:在本研究中,评估了三种微生物DNA富集技术:1。一系列的离心步骤,2。2 .使用NEBNext®微生物组DNA富集试剂盒富集微生物DNA;全基因组扩增遵循先前的富集策略。还包括无治疗对照组和全基因组扩增对照组。本研究收集了3例接受功能性鼻内窥镜鼻窦手术治疗慢性鼻窦炎(CRS)的成年患者的拭子样本。采用Illumina HiSeq对端霰弹枪宏基因组测序,采用Illumina MiSeq对细菌16S rRNA基因扩增子测序,评估细菌群落组成。
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Assessment of microbial DNA enrichment techniques from sino-nasal swab samples for metagenomics
Methods: In this study, three techniques were assessed for microbial DNA enrichment: 1. A series of centrifugation steps, 2. Enrichment of microbial DNA using the NEBNext® Microbiome DNA Enrichment kit, and 3. Whole-genome amplification following the previous enrichment strategies. A no-treatment control and a whole-genome amplified control were also included. Swab samples from three adult patients undergoing functional endoscopic sinus surgery for chronic rhinosinusitis (CRS) were collected intraoperatively for this study. Paired-end shotgun metagenome sequencing was conducted using Illumina HiSeq and bacterial 16S rRNA gene amplicon sequencing using Illumina MiSeq to assess bacterial community composition.
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