Tayo Micheal Anjuwon, Joseph Olorunmola Ehinmidu, Kola Matthew Anigo, Dorcas Bolanle James
{"title":"恶性疟原虫和伯氏疟原虫分离株对所选抗疟药物的体外抗疟敏感性、草甘膦叶提取物的柱色谱亚组分以及SF8的FTIR和GCNS","authors":"Tayo Micheal Anjuwon, Joseph Olorunmola Ehinmidu, Kola Matthew Anigo, Dorcas Bolanle James","doi":"10.21315/tlsr2023.34.2.14","DOIUrl":null,"url":null,"abstract":"<p><p>Malaria still remains a life-threatening parasitic disease with universal targets set for control and elimination. This study aimed to evaluate the <i>in vitro</i> antimalarial susceptibility of <i>Plasmodium falciparum</i> isolates and <i>Plasmodium berghei</i> to selected antimalarial agents and column chromatographic subfractions of <i>Glyphaea brevis</i> leaves extract and FTIR and GCMS of SF8. Trager and Jensen as well as World Health Organisation (WHO) standardised <i>in vitro</i> micro-test system methods were used to determine susceptibility on the patients' blood samples; Column chromatographic procedure was carried out to obtain 11 pooled fractions; FTIR and GCMS were used to determine functional groups and phytochemicals respectively. <i>In vitro</i> anti-plasmodial activity against <i>P. falciparum</i> clinical isolates had IC<sub>50</sub> range of 1.03 μg/mL-7.63 μg/mL while their IC<sub>50</sub> against <i>P. berghei</i> ranges from 4.32 μg/mL-7.89 μg/mL. Subfraction 8 (SF8) had the least IC<sub>50</sub> of 4.32 μg/mL. The FTIR spectrum showed the presence of isoprenoid, alcohol, phenol, alkane, alkenes, ester, carboxylic acids, aromatics and nitro compounds while GCMS identified dodecanoic acid, methyl ester; carotol; hexadecanoic acid, methyl ester; 9-octadecenoic acid (Z)-, methyl ester (oleic acid); methyl stearate; heptadecanoic acid, 16-methyl-, methyl ester; all with their antimalarial reported activities. In conclusion, <i>G. brevis</i> has a great potential for drug development against malaria parasite since it inhibited schizont growth and possesses phytocompounds with antimalarial report.</p>","PeriodicalId":23477,"journal":{"name":"Tropical life sciences research","volume":null,"pages":null},"PeriodicalIF":1.1000,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10735266/pdf/","citationCount":"0","resultStr":"{\"title\":\"<i>In Vitro</i> Antimalarial Susceptibility of <i>Plasmodium falciparum</i> and <i>Plasmodium berghei</i> Isolates to Selected Antimalarial Agents, Column Chromatographic Subfractions of <i>Glyphaea brevis</i> Leaves Extract and FTIR and GCMS of SF8.\",\"authors\":\"Tayo Micheal Anjuwon, Joseph Olorunmola Ehinmidu, Kola Matthew Anigo, Dorcas Bolanle James\",\"doi\":\"10.21315/tlsr2023.34.2.14\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Malaria still remains a life-threatening parasitic disease with universal targets set for control and elimination. This study aimed to evaluate the <i>in vitro</i> antimalarial susceptibility of <i>Plasmodium falciparum</i> isolates and <i>Plasmodium berghei</i> to selected antimalarial agents and column chromatographic subfractions of <i>Glyphaea brevis</i> leaves extract and FTIR and GCMS of SF8. Trager and Jensen as well as World Health Organisation (WHO) standardised <i>in vitro</i> micro-test system methods were used to determine susceptibility on the patients' blood samples; Column chromatographic procedure was carried out to obtain 11 pooled fractions; FTIR and GCMS were used to determine functional groups and phytochemicals respectively. <i>In vitro</i> anti-plasmodial activity against <i>P. falciparum</i> clinical isolates had IC<sub>50</sub> range of 1.03 μg/mL-7.63 μg/mL while their IC<sub>50</sub> against <i>P. berghei</i> ranges from 4.32 μg/mL-7.89 μg/mL. Subfraction 8 (SF8) had the least IC<sub>50</sub> of 4.32 μg/mL. The FTIR spectrum showed the presence of isoprenoid, alcohol, phenol, alkane, alkenes, ester, carboxylic acids, aromatics and nitro compounds while GCMS identified dodecanoic acid, methyl ester; carotol; hexadecanoic acid, methyl ester; 9-octadecenoic acid (Z)-, methyl ester (oleic acid); methyl stearate; heptadecanoic acid, 16-methyl-, methyl ester; all with their antimalarial reported activities. In conclusion, <i>G. brevis</i> has a great potential for drug development against malaria parasite since it inhibited schizont growth and possesses phytocompounds with antimalarial report.</p>\",\"PeriodicalId\":23477,\"journal\":{\"name\":\"Tropical life sciences research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2023-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10735266/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Tropical life sciences research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21315/tlsr2023.34.2.14\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/7/21 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical life sciences research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21315/tlsr2023.34.2.14","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/7/21 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"BIOLOGY","Score":null,"Total":0}
In Vitro Antimalarial Susceptibility of Plasmodium falciparum and Plasmodium berghei Isolates to Selected Antimalarial Agents, Column Chromatographic Subfractions of Glyphaea brevis Leaves Extract and FTIR and GCMS of SF8.
Malaria still remains a life-threatening parasitic disease with universal targets set for control and elimination. This study aimed to evaluate the in vitro antimalarial susceptibility of Plasmodium falciparum isolates and Plasmodium berghei to selected antimalarial agents and column chromatographic subfractions of Glyphaea brevis leaves extract and FTIR and GCMS of SF8. Trager and Jensen as well as World Health Organisation (WHO) standardised in vitro micro-test system methods were used to determine susceptibility on the patients' blood samples; Column chromatographic procedure was carried out to obtain 11 pooled fractions; FTIR and GCMS were used to determine functional groups and phytochemicals respectively. In vitro anti-plasmodial activity against P. falciparum clinical isolates had IC50 range of 1.03 μg/mL-7.63 μg/mL while their IC50 against P. berghei ranges from 4.32 μg/mL-7.89 μg/mL. Subfraction 8 (SF8) had the least IC50 of 4.32 μg/mL. The FTIR spectrum showed the presence of isoprenoid, alcohol, phenol, alkane, alkenes, ester, carboxylic acids, aromatics and nitro compounds while GCMS identified dodecanoic acid, methyl ester; carotol; hexadecanoic acid, methyl ester; 9-octadecenoic acid (Z)-, methyl ester (oleic acid); methyl stearate; heptadecanoic acid, 16-methyl-, methyl ester; all with their antimalarial reported activities. In conclusion, G. brevis has a great potential for drug development against malaria parasite since it inhibited schizont growth and possesses phytocompounds with antimalarial report.
期刊介绍:
Tropical Life Sciences Research (TLSR) formerly known as Journal of Bioscience seeks to publish relevant ideas and knowledge addressing vital life sciences issues in the tropical region. The Journal’s scope is interdisciplinary in nature and covers any aspects related to issues on life sciences especially from the field of biochemistry, microbiology, biotechnology and animal, plant, environmental, biomedical and pharmaceutical sciences. TLSR practices double blind peer review system to ensure and maintain the good quality of articles published in this journal. Two issues are published annually in printed and electronic form. TLSR also accepts review articles, experimental papers and short communications. The Chief Editor would like to invite researchers to use this journal as a mean to rapidly promote their research findings.