纳米颗粒和微颗粒冻干同种异体骨包被纳米纤维支架对人间充质干细胞形态、粘附和增殖的影响

Q2 Biochemistry, Genetics and Molecular Biology Iranian Biomedical Journal Pub Date : 2022-04-27 DOI:10.52547/ibj.26.3.193
Shabnam Aghayan, E. Seyedjafari, Shadi Hamidi
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引用次数: 0

摘要

背景:纳米纤维膜上冷冻干燥的同种异体骨纳米颗粒可能是骨再生的理想支架。本研究旨在评估人骨髓间充质干细胞在增殖和粘附于PLLA纳米纤维膜上的纳米和微粒FDBA支架方面的生物学行为。方法:采用静电纺丝法合成PLLA纳米纤维支架。采用机械法制备了FDBA纳米粒子。将FDBA纳米粒子和微粒负载在PLLA纳米纤维膜表面。将n-FDBA/PLLA、FDBA/PLA、PLLA和对照四组共64个支架样品置于24孔聚苯乙烯组织培养板中;每组分配16口井。数据采用单因素方差分析和Bonferroni检验进行分析。结果:与微粒组相比,纳米颗粒组的MSCs在第五天的增殖率显著更高(p=0.034)。24小时的细胞形态评估显示,与其他组相比,纳米粒子组的纺锤形细胞具有更多的附属物。结论:与FDBA/PLLA相比,n-FDBA/PLA支架上的MSCs在形态上更活跃、更平坦,具有更多的细胞附属物。在体外培养的MSCs的增殖、附着和形态方面,纳米颗粒支架似乎优于微粒支架。
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Effects of Nanofiber Scaffolds Coated with Nanoparticulate and Microparticulate Freeze Dried Bone Allograft on the Morphology, Adhesion, and Proliferation of Human Mesenchymal Stem Cells
Background: Freeze dried bone allograft nanoparticles on a nanofiber membrane may serve as an ideal scaffold for bone regeneration. This study aimed to assess the biological behavior of human MSCs in terms of proliferation and adhesion to nanoparticulate and microparticulate FDBA scaffolds on PLLA nanofiber membrane. Methods: In this experimental study, PLLA nanofiber scaffolds were synthesized by the electrospinning method. The FDBA nanoparticles were synthesized mechanically. The FDBA nanoparticles and microparticles were loaded on the surface of PLLA nanofiber membrane. A total of 64 scaffold samples in four groups of n-FDBA/PLLA, FDBA/PLLA, PLLA and control were placed in 24-well polystyrene tissue culture plates; 16 wells were allocated to each group. Data were analyzed using one-way ANOVA and Bonferroni test. Results: The proliferation rate of MSCs was significantly higher in the nanoparticulate group compared to the microparticulate group at five days (p = 0.034). Assessment of cell morphology at 24 hours revealed spindle-shaped cells with a higher number of appendages in the nanoparticulate group compared to other groups. Conclusion: MSCs on n-FDBA/PLLA scaffold were morphologically more active and flatter with a higher number of cellular appendages, as compared to FDBA/PLLA. It seems that the nanoparticulate scaffold is superior to the microparticulate scaffold in terms of proliferation, attachment, and morphology of MSCs in vitro.
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来源期刊
Iranian Biomedical Journal
Iranian Biomedical Journal Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
3.20
自引率
0.00%
发文量
42
审稿时长
8 weeks
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