刺山柑水醇提取物对小鼠面部福尔马林试验模型的镇痛作用及组织病理学影响的实验研究

Electronic Physician Pub Date : 2019-08-25 DOI:10.19082/7567
Arezou Rayyani, S. Seifi, Roja Askian, M. Ashrafpour, S. Kazemi, A. Moghadamnia, H. Gholinia
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引用次数: 0

摘要

背景:口面部疼痛是一种炎症性疼痛,通常用皮质类固醇治疗,有许多副作用。目的:本研究的目的是研究刺山柑(Capparis spinosa)水醇提取物首次在口腔面部的镇痛作用的临床和组织学特征。方法:本实验研究于2018年在巴勃尔医学科学大学进行。36只雄性Wistar小鼠被分为6组:第一组接受生理盐水,第二组接受地塞米松,四组接受不同剂量(10、20、50、100mg/kg)的刺山柑提取物。在福尔马林试验中,将1%的福尔马林溶液注射到嘴唇的右侧粘膜下层和鼻子的外侧区域。在注射后的60分钟内,以5分钟的阻滞记录疼痛强度。记录跳跃对疼痛的剂量效应。对小鼠实施安乐死,并对口腔区域进行活检,并用苏木精-伊红、甲苯胺蓝和刚果红染色。数据采用重复测量方差分析和IBM-SPSS版本20的t检验进行分析。结果:与生理盐水组相比,刺五加具有镇痛作用(p0.999)。组织病理学检查显示,刺五加100mg/kg剂量时,上皮、纤维和肌肉组织密度最高,炎症浸润最低。结论:刺山柑水醇提取物具有剂量依赖性的镇痛活性,刺山柑诱导的镇痛作用可能至少部分由抗炎作用介导。
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Pain modulation and Histopathological effects of the Hydroalcoholic extract of Capparis spinosa on mice model of Orofacial formalin test: an experimental study
Background: Orofacial pain is a form of inflammatory pain usually treated with corticosteroids, which have many side effects. Objective: The aim of this study was to investigate the clinical and histological profile of antinociceptive effects of hydroalcoholic extract of caper (Capparis spinosa) for the first time in the orofacial region. Methods: This experimental study was carried out at Babol University of Medical Sciences in 2018. Thirty-six male Wistar mice were divided into 6 groups: The first group received saline, the second group received dexamethazone, and four groups received different doses (10, 20, 50, 100 mg/kg) of caper extract. In the formalin test, 1% formalin solution was injected into the right submucosal layer of the lip and lateral area of the nose. Pain intensities were recorded at 5-min blocks for 60 min after injection. Dose effect of caper on pain was recorded. The mice were euthanized and the oral area was biopsied and stained with Hematoxylin-eosin, Toluidine blue, and Congo red. Data were analyzed using repeated measures ANOVA and t-test by IBM-SPSS version 20. Results: Caper produced antinociceptive effects in comparison with saline groups (p<0.001). Dose effect on pain was significant (p<0.001). The highest antinociceptive effect was observed in the caper group receiving 100 mg/kg of agent 15-20 minutes after injection. The highest pain level was observed in the group that received 20 mg/kg of caper (p<0.001). Dexamethazone antinociceptive effect was greater than that of the saline and the dose of 20 mg/kg of caper (p<0.001). Antinociceptive effects in two groups (100 mg caper and dexamethazone) were equal (p>0.999). Histopathologic examination revealed the highest thickness of epithelium, fibrous, and muscular tissue density and the lowest inflammatory infiltration at the dose of 100 mg/kg of caper. Conclusion: The results of the present study suggest that hydroalcoholic extract of caper possesses antinociceptive activity in a dose-dependent manner and caper-induced antinociception might be mediated, at least in part, by anti-inflammatory effects.
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