T. O. S. Tjia, K. Meitha, P. Septiani, R. Awaludin, D. Sumardi
{"title":"细胞外自身DNA诱导水稻根系生长的局部抑制,调节活性氧的产生和基因表达","authors":"T. O. S. Tjia, K. Meitha, P. Septiani, R. Awaludin, D. Sumardi","doi":"10.32615/bp.2022.037","DOIUrl":null,"url":null,"abstract":"The capacity of extracellular self-DNA (esDNA) to inhibit growth is getting more research attention as this could be explored for several purposes, including the development of specific bioherbicides. While the inhibitory effect has been studied in several dicotyledon species, little is known about the effects and subsequent signaling processes in monocots. Here, we measured the growth, counted the number of lateral and crown roots, determined greenness index, quantified the production of O 2.- and H 2 O 2 , and determined the expressions of genes encoding antioxidant enzymes ( SOD s and CAT s) in rice ( Oryza sativa L.), a model plant of monocots. After 7 d of germination, rice roots were exposed to 0, 75, and 150 µg cm -3 of esDNA. Inhibitory effect was found to be negatively correlated to esDNA concentration, as indicated by the length of primary roots. Interestingly, this negative effect was only observed in the directly exposed organ (root) but not in the length of shoot or fresh mass of the whole seedling. The percentage of greenness index of leaves and number of crown and lateral roots were also similar across treatments. However, esDNA exposure to root increased production of O 2.- and H 2 O 2 in the root. At the molecular level, the response was characterized by the decreased expression of the antioxidant genes SOD 3, CAT B, and CAT C. These findings suggest that esDNA inhibits rice growth locally in, e.g. in treated roots, and the responses involve increased production of ROS and suppression of antioxidants. This study could be the basis for determining the combination of concentration and period of exposure that might significantly inhibit total growth of monocot weeds with a minimum effect on the crop.","PeriodicalId":8912,"journal":{"name":"Biologia Plantarum","volume":null,"pages":null},"PeriodicalIF":0.8000,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Extracellular self-DNA induces local inhibition of growth, regulates production of reactive oxygen species, and gene expression in rice roots\",\"authors\":\"T. O. S. Tjia, K. Meitha, P. Septiani, R. Awaludin, D. Sumardi\",\"doi\":\"10.32615/bp.2022.037\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The capacity of extracellular self-DNA (esDNA) to inhibit growth is getting more research attention as this could be explored for several purposes, including the development of specific bioherbicides. While the inhibitory effect has been studied in several dicotyledon species, little is known about the effects and subsequent signaling processes in monocots. Here, we measured the growth, counted the number of lateral and crown roots, determined greenness index, quantified the production of O 2.- and H 2 O 2 , and determined the expressions of genes encoding antioxidant enzymes ( SOD s and CAT s) in rice ( Oryza sativa L.), a model plant of monocots. After 7 d of germination, rice roots were exposed to 0, 75, and 150 µg cm -3 of esDNA. Inhibitory effect was found to be negatively correlated to esDNA concentration, as indicated by the length of primary roots. Interestingly, this negative effect was only observed in the directly exposed organ (root) but not in the length of shoot or fresh mass of the whole seedling. The percentage of greenness index of leaves and number of crown and lateral roots were also similar across treatments. However, esDNA exposure to root increased production of O 2.- and H 2 O 2 in the root. At the molecular level, the response was characterized by the decreased expression of the antioxidant genes SOD 3, CAT B, and CAT C. These findings suggest that esDNA inhibits rice growth locally in, e.g. in treated roots, and the responses involve increased production of ROS and suppression of antioxidants. This study could be the basis for determining the combination of concentration and period of exposure that might significantly inhibit total growth of monocot weeds with a minimum effect on the crop.\",\"PeriodicalId\":8912,\"journal\":{\"name\":\"Biologia Plantarum\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2023-01-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biologia Plantarum\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.32615/bp.2022.037\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biologia Plantarum","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.32615/bp.2022.037","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Extracellular self-DNA induces local inhibition of growth, regulates production of reactive oxygen species, and gene expression in rice roots
The capacity of extracellular self-DNA (esDNA) to inhibit growth is getting more research attention as this could be explored for several purposes, including the development of specific bioherbicides. While the inhibitory effect has been studied in several dicotyledon species, little is known about the effects and subsequent signaling processes in monocots. Here, we measured the growth, counted the number of lateral and crown roots, determined greenness index, quantified the production of O 2.- and H 2 O 2 , and determined the expressions of genes encoding antioxidant enzymes ( SOD s and CAT s) in rice ( Oryza sativa L.), a model plant of monocots. After 7 d of germination, rice roots were exposed to 0, 75, and 150 µg cm -3 of esDNA. Inhibitory effect was found to be negatively correlated to esDNA concentration, as indicated by the length of primary roots. Interestingly, this negative effect was only observed in the directly exposed organ (root) but not in the length of shoot or fresh mass of the whole seedling. The percentage of greenness index of leaves and number of crown and lateral roots were also similar across treatments. However, esDNA exposure to root increased production of O 2.- and H 2 O 2 in the root. At the molecular level, the response was characterized by the decreased expression of the antioxidant genes SOD 3, CAT B, and CAT C. These findings suggest that esDNA inhibits rice growth locally in, e.g. in treated roots, and the responses involve increased production of ROS and suppression of antioxidants. This study could be the basis for determining the combination of concentration and period of exposure that might significantly inhibit total growth of monocot weeds with a minimum effect on the crop.
期刊介绍:
BIOLOGIA PLANTARUM is an international journal for experimental botany. It publishes original scientific papers and brief communications, reviews on specialized topics, and book reviews in plant physiology, plant biochemistry and biophysics, physiological anatomy, ecophysiology, genetics, molecular biology, cell biology, evolution, and pathophysiology. All papers should contribute substantially to the current level of plant science and combine originality with a potential general interest. The journal focuses on model and crop plants, as well as on under-investigated species.