真皮细胞外基质对facetem植入物的反应:一项随机对照实验研究

G. Mogilnaya, E. V. Fomicheva
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引用次数: 0

摘要

背景现代再生医学的一个主要概念是利用自身的身体资源,通过形成“活支架”来重塑器官和组织。产生细胞外基质(ECM)的成纤维细胞在真皮支架的形成中发挥着关键作用。目的:研究在Facetem填充剂给药下,成纤维细胞活化介导真皮ECM原纤维成分合成的机制。方法。实验试验在Wistar雄性大鼠(72只动物)中进行。动物皮下注射0.05 mL Facetem(韩国)。填料是一种含钙产品,通过Lattice孔隙技术的结构微球特性逐渐降解。在第1、2、3和5个月的第1周和第2周对生物材料进行取样。将组织石蜡包埋在标准组织学测定中并在偏振显微镜下用Mallory三色染色、Picrosirius红染色以及用I型胶原进行免疫组织化学染色,III和弹性蛋白抗体(Abcam)。结果。真皮和填充区中的胶原分布表明,在Facetem填充物放置的第2周,胶原产生,随后合成基质体积增加到I型胶原的4.39±0.7和III型胶原的3.9±0.2(p<0.05)。成纤维细胞的合成活性在第3个月降低,尽管即使在第5个月结束时胶原生产仍保持在控制之上。在真皮中注射填充剂的第2周,弹性蛋白的合成也开始,并在第3个月生长。结论:Facetem填充剂的存在会引发真皮中的异物炎症反应。这一多因素过程始于蛋白质吸附,进而进行真皮细胞的募集和成纤维细胞和巨噬细胞的调节。这些细胞类型的激活诱导新胶原生成,这涉及真皮中细胞外基质的合成和扩张。
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Dermal extracellular matrix response to facetem implant: a randomised controlled experimental study
Background. A leading concept in modern regenerative medicine is the perspective of using own body resources to remodel organs and tissues via the formation of “living scaffold”. A pivotal role in the formation of dermal scaffold is played by fibroblasts that produce extracellular matrix (ECM).Aim. A study of the fibroblast activation mechanism mediating synthesis of the dermal ECM fibrillar component under the Facetem filler administration.Methods. The experimental trial was conducted in Wistar male rats (72 animals). Animals had 0.05 mL Facetem (Korea) injections subdermally. The filler is a calcium-containing product featuring gradual degradation delivered through structural microspheric properties of the Lattice-pore technology. Biological material was sampled at weeks 1 and 2 of months 1, 2, 3 and 5. Tissues were paraffin-embedded in standard histological assays and stained with Mallory’s trichrome, Picrosirius red in polarisation microscopy and immunohistochemistry with collagen types I, III and elastin antibodies (Abcam).Results. Collagen distribution in dermis and the filling zone suggests that collagen production occurs by week 2 of the Facetem filler placement followed by an increase in synthesised matrix volume to 4.39 ± 0.7 for collagen type I and 3.9 ± 0.2 for collagen type III (p < 0.05). The synthetic activity of fi broblasts reduces by month 3, albeit with collagen production remaining above control even by the end of month 5. Elastin synthesis also initiates by week 2 of the filler injection in dermis and grows by month 3.Conclusion. The presence of Facetem filler triggers a foreign body inflammatory response in dermis. This multifactorial process initiates with protein adsorption proceeding to dermal cell recruitment and modulation of fibroblasts and macrophages. Activation of these cell types induces neocollagenesis entailing the extracellular matrix synthesis and expansion in dermis.
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CiteScore
0.10
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发文量
37
审稿时长
8 weeks
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