家蝇胸段免疫原在单次放射免疫扩散试验检测山羊免疫球蛋白g水平中的应用

L. Rumokoy, W. L. Toar
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引用次数: 0

摘要

本研究的目的是检测家蝇胸免疫原(ITMd)处理山羊的IgG水平。我们在广泛的处理下饲养了12只两个月大的山羊,分成两组进行比较实验设计。第一组(R0)中的动物作为对照,不注射免疫原,第二组(R1)是接受每人皮下注射10mL胸部抗原的治疗组。观察到的参数是IgG(免疫球蛋白-G)抗体血清水平。山羊免疫球蛋白的定量是使用单一放射免疫扩散(SRID)技术进行的。从两组获得的数据用t-研究检验进行分析。结果表明,治疗组山羊的总IgG抗体血清显著高于对照组(P<0.05),这可能是由于胸部家蝇免疫原反应增加了实验山羊的抗体合成所致。我们得出的结论是,皮下注射10µL的家蝇胸部免疫原治疗可以显著增加山羊血中的IgG抗体,这是使用单一放射免疫扩散法检测到的。
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Application of Thoracic Immunogen of Musca domestica on Immunoglobulin-G Level of Goats Detected Through a Single Radial Immuno-Diffusion Test
The purpose of this study was to detect the IgG level of goats treated with immunogen thoracic of Musca domestica (ITMd). We reared twelve young goats aged two months old under extensive treatments, divided into two equal groups for a comparison experimental design. The animals in the first group (R0) served as the control without immunogen injection, and the second group (R1) was the treatment group receiving a subcutaneous injection of 10 mL of thoracic antigens per head. The parameter observed was IgG (immunoglobulin-G) antibody serum level. The quantification of goat immunoglobulins was carried out using a single radial immuno-diffusion (SRID) technique. The data obtained from the two groups were analyzed with a t-student test. The results showed that the total IgG antibody serum of goats in the treatment group was significantly higher than that of the control group (P< 0.05). This difference may be due to the thoracic M. domestica immunogen response which increased antibody synthesis of experiment goats. We concluded that the treatment of thoracic immunogen of M. domestica at a level of 10 µL injected subcutaneously could significantly increase IgG antibodies in goat blood which were detected using a single radial immuno-diffusion method.
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发文量
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审稿时长
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