Biological study of platelet-rich plasma-Cage complex on reconstruction of cervical vertebrae

Objective To explore the ability of reconstructing cervical vertebrae of platelet-rich plasma (PRP) and CAGE with the compound of humeral cortical ring allograft (HCA) packed with cancellous allogenic bone (CALB), and find some evidence to support the clinical practice of PRP-CAGE complex on reconstruction of vertebral body. Methods The animal models of a cervical vertebrae defect were created by surgery in 26 New Zealand white rabbits. The A group were treated with the compound of PRP-CAGE; the B group with autogenous iliac crest. Observations were made by gross, X-ray, histopathological, scanning electron microscope (SEM) examination and the effect were determined by biomechanics at different periods postoperatively.Statistical methods is t Test, there was significant difference in statistics (P<0.05). Results The gross and X-ray examination showed that spine fusion in A and B group at 2 months. Histological analysis exhibited scattered bone island and bone trabecula formation at 1 month, many mature bone matrix and bone marrow cavity formation at 2 months; the SEM also showed that there were many new bone and osteoblast formed in tow groups. The flexion values on the tests of the 50 N load-strain in flexion, extension were 52.33±3.10, 53.98±3.80 in 2 groups, The extension values were 42.63±2.80, 47.23±2.30 in 2 groups. The effect of biomechanics had no statistically in tow groups (t=0.583, 2.199, P>0.05). Conclusion The compound of PRP-Cage possesses much high bone inductive potentialities and biomechanic stability. PRP may be associated with the role of multiple growth factors in regulating cell function, improving the tissue microenvironment, and promoting intervertebral fusion. Key words: Autologous platelet-rich plasma; Transplantation, homologous; Spinal fusion; Cervical vertebrae