Raluca-Ioana Ștefan-van Staden, Ruxandra-Maria Ilie-Mihai, M. Coroş, S. Pruneanu
{"title":"生物样品中MLH1、MSH2、MSH6、PMS2和KRAS的分子识别和定量","authors":"Raluca-Ioana Ștefan-van Staden, Ruxandra-Maria Ilie-Mihai, M. Coroş, S. Pruneanu","doi":"10.1149/2754-2726/ac9740","DOIUrl":null,"url":null,"abstract":"Two stochastic sensors based on modification of nitrogen and boron dopped exfoliated graphene with a complex of protoporphyrin and cobalt, were used for molecular recognition and quantification of MLH1, MSH2, MSH6, PMS2 and KRAS biomarkers in biological samples (whole blood, urine, saliva, tumoral tissue). Limits of determination of fg ml−1 magnitude order and broad linear concentration ranges favorized their determination from very low to higher concentrations in biological samples. The student t-test showed that there is no significant difference between the results obtained by utilizing the two microsensors for screening tests, at 99% confidence level, the results obtained being lowr than the tabulated value.","PeriodicalId":72870,"journal":{"name":"ECS sensors plus","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"6","resultStr":"{\"title\":\"Molecular Recognition and Quantification of MLH1, MSH2, MSH6, PMS2, and KRAS in Biological Samples\",\"authors\":\"Raluca-Ioana Ștefan-van Staden, Ruxandra-Maria Ilie-Mihai, M. Coroş, S. Pruneanu\",\"doi\":\"10.1149/2754-2726/ac9740\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Two stochastic sensors based on modification of nitrogen and boron dopped exfoliated graphene with a complex of protoporphyrin and cobalt, were used for molecular recognition and quantification of MLH1, MSH2, MSH6, PMS2 and KRAS biomarkers in biological samples (whole blood, urine, saliva, tumoral tissue). Limits of determination of fg ml−1 magnitude order and broad linear concentration ranges favorized their determination from very low to higher concentrations in biological samples. The student t-test showed that there is no significant difference between the results obtained by utilizing the two microsensors for screening tests, at 99% confidence level, the results obtained being lowr than the tabulated value.\",\"PeriodicalId\":72870,\"journal\":{\"name\":\"ECS sensors plus\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-10-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ECS sensors plus\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1149/2754-2726/ac9740\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ECS sensors plus","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1149/2754-2726/ac9740","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Molecular Recognition and Quantification of MLH1, MSH2, MSH6, PMS2, and KRAS in Biological Samples
Two stochastic sensors based on modification of nitrogen and boron dopped exfoliated graphene with a complex of protoporphyrin and cobalt, were used for molecular recognition and quantification of MLH1, MSH2, MSH6, PMS2 and KRAS biomarkers in biological samples (whole blood, urine, saliva, tumoral tissue). Limits of determination of fg ml−1 magnitude order and broad linear concentration ranges favorized their determination from very low to higher concentrations in biological samples. The student t-test showed that there is no significant difference between the results obtained by utilizing the two microsensors for screening tests, at 99% confidence level, the results obtained being lowr than the tabulated value.