严重急性呼吸系统综合征冠状病毒2型抗体表位定位

Q3 Medicine ImmunoHorizons Pub Date : 2022-06-01 DOI:10.4049/immunohorizons.2200030
Jiaan Yang, Peng Zhang, Wenxiang Cheng, Gang Wu, Q. Niu, Lan Yang, Shun Luo, Xianghua Lin, Lianshan Zhang
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引用次数: 1

摘要

由于蛋白质三维结构的复杂性,严重急性呼吸综合征冠状病毒2 (SARS-CoV-2)与抗体相互作用的表位定位具有挑战性。应用蛋白结构指纹图谱技术对44株具有三维结构复合物的SARS-CoV-2抗体进行表位定位。结果确定了表位在SARS-CoV-2上的分布,以及来自抗体的6种cdr的模式如何参与中和。此外,残基-残基识别结果显示,某些残基在SARS-CoV-2与抗体的界面上具有较高的频率,其活性与界面上残基的物理化学性质相关。因此,表位定位为开发基于表位的抗体、疫苗和诊断试剂设计提供了重要的线索信息。这是一个结构数据分析的生物信息学项目;没有使用动物或细胞。
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Severe Acute Respiratory Syndrome Coronavirus 2 Epitope Mapping for Antibodies
Epitope mapping of the interactions between severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and Abs is challenging because of complexity in protein three-dimensional structures. Protein structure fingerprint technology was applied for epitope mapping of 44 SARS-CoV-2 Abs with three-dimensional structure complexes. The results defined how the epitopes were distributed on SARS-CoV-2 and how the patterns of six CDRs from Abs participated in neutralization. Also, the residue–residue recognition revealed that certain residues had higher frequencies on the interfaces between SARS-CoV-2 and Abs, and the activity correlated with the physicochemical properties of the residues at the interface. Thus, epitope mapping provides significant lead information for development of epitope-based designs for Abs, vaccines, and diagnostic reagents. This is a bioinformatics project of structural data analysis; no animals or cells were used.
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CiteScore
3.70
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0.00%
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审稿时长
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