Honggang Guo, S. Feng, Yuebin Zhou, Fang-Lian Yao, Yinzhong Liu, Zhi Chen, Y. Liang, Tao Wang
{"title":"表面修饰组织工程支架预防脊髓界面骨溶解机制的初步研究","authors":"Honggang Guo, S. Feng, Yuebin Zhou, Fang-Lian Yao, Yinzhong Liu, Zhi Chen, Y. Liang, Tao Wang","doi":"10.3760/CMA.J.ISSN.1001-9030.2019.12.038","DOIUrl":null,"url":null,"abstract":"Objective \nTo evaluate the interrelationship between intervertebral osteolysis and inflammatory or osteoclastic gene expression. \n \n \nMethods \nAdipose derived stromal cells (ADSCs) were osteoinduced, conventioanl tissue-engineered (TE) and surface-modified TE cage constructs were establisehd. Anterior cervical fusion model of goat was established. Implantation was carried out according to following six group: surface-modified TE cage group [Nd: YAG+ RGD+ β- tricalcium phosphate (β-TCP)/chitosan (CS)/polycaprolactone (PCL) cage+ ADSCs], TE cage group (β-TCP/CS/PCL cage+ ADSCs), non-modified β-TCP/CS/PCL cage, hydroxyapatite (HA) cage, titanium and bone allograft cage. Three-dimensional CT was used to observe morphology changing, bone mineralization ratio, new bone mension and osteoblatic quantity, interleukin-6 (IL-6), metalloprotease-1 (MMP-1), tumor necrosis factor-α (TNF-α) and tartrate resistant acid phosphatase (TRAP), and osteoprotein (OPG) gene expression were detected at 2, 4, 8, 12, 16, 20, 24, 28 and 36 weeks. Biomechanical property was also detected at different interval. \n \n \nResults \nCompared with other groups, implant subsidence and migration were not found in surface-modified TE cage group. Meanwhile, new bone prolifreated remarkably with satisfactory data of bone mineralization ratio, and new bone mension or osteoblatic quantity [(63.9±1.3)%, (76.54±1.8)%, (73.8±2.3) cells]. IL-6, MMP-1, TNF-α and TRAP gene downregulated (0.09±0.01, 0.13±0.02, 0.08±0.01). Conversely, Runx2 and OPG gene upregulated (0.73±0.13, 0.57±0.16). In addition, data of bone strength and rang of motion in surface-modified TE cage group were significantly higher than those of other groups [(4.38±0.25) Nm/degree, (6.55±0.19)°]. \n \n \nConclusion \nInvertebral osteolysis can be effectively controlled by surface-modified TE approach, and this provides a potential therapy for implant aseptic loosening. \n \n \nKey words: \nOsteolysis; Cage; Surface modification; Tissue engineering; Interface","PeriodicalId":10065,"journal":{"name":"中华实验外科杂志","volume":"36 1","pages":"2250-2253"},"PeriodicalIF":0.0000,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The preliminary investigation on mechanism of preventing spinal interface osteolysis via surface-modified tissue-engineered cage\",\"authors\":\"Honggang Guo, S. Feng, Yuebin Zhou, Fang-Lian Yao, Yinzhong Liu, Zhi Chen, Y. Liang, Tao Wang\",\"doi\":\"10.3760/CMA.J.ISSN.1001-9030.2019.12.038\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective \\nTo evaluate the interrelationship between intervertebral osteolysis and inflammatory or osteoclastic gene expression. \\n \\n \\nMethods \\nAdipose derived stromal cells (ADSCs) were osteoinduced, conventioanl tissue-engineered (TE) and surface-modified TE cage constructs were establisehd. Anterior cervical fusion model of goat was established. Implantation was carried out according to following six group: surface-modified TE cage group [Nd: YAG+ RGD+ β- tricalcium phosphate (β-TCP)/chitosan (CS)/polycaprolactone (PCL) cage+ ADSCs], TE cage group (β-TCP/CS/PCL cage+ ADSCs), non-modified β-TCP/CS/PCL cage, hydroxyapatite (HA) cage, titanium and bone allograft cage. Three-dimensional CT was used to observe morphology changing, bone mineralization ratio, new bone mension and osteoblatic quantity, interleukin-6 (IL-6), metalloprotease-1 (MMP-1), tumor necrosis factor-α (TNF-α) and tartrate resistant acid phosphatase (TRAP), and osteoprotein (OPG) gene expression were detected at 2, 4, 8, 12, 16, 20, 24, 28 and 36 weeks. Biomechanical property was also detected at different interval. \\n \\n \\nResults \\nCompared with other groups, implant subsidence and migration were not found in surface-modified TE cage group. Meanwhile, new bone prolifreated remarkably with satisfactory data of bone mineralization ratio, and new bone mension or osteoblatic quantity [(63.9±1.3)%, (76.54±1.8)%, (73.8±2.3) cells]. IL-6, MMP-1, TNF-α and TRAP gene downregulated (0.09±0.01, 0.13±0.02, 0.08±0.01). Conversely, Runx2 and OPG gene upregulated (0.73±0.13, 0.57±0.16). In addition, data of bone strength and rang of motion in surface-modified TE cage group were significantly higher than those of other groups [(4.38±0.25) Nm/degree, (6.55±0.19)°]. \\n \\n \\nConclusion \\nInvertebral osteolysis can be effectively controlled by surface-modified TE approach, and this provides a potential therapy for implant aseptic loosening. \\n \\n \\nKey words: \\nOsteolysis; Cage; Surface modification; Tissue engineering; Interface\",\"PeriodicalId\":10065,\"journal\":{\"name\":\"中华实验外科杂志\",\"volume\":\"36 1\",\"pages\":\"2250-2253\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-12-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中华实验外科杂志\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2019.12.038\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中华实验外科杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2019.12.038","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The preliminary investigation on mechanism of preventing spinal interface osteolysis via surface-modified tissue-engineered cage
Objective
To evaluate the interrelationship between intervertebral osteolysis and inflammatory or osteoclastic gene expression.
Methods
Adipose derived stromal cells (ADSCs) were osteoinduced, conventioanl tissue-engineered (TE) and surface-modified TE cage constructs were establisehd. Anterior cervical fusion model of goat was established. Implantation was carried out according to following six group: surface-modified TE cage group [Nd: YAG+ RGD+ β- tricalcium phosphate (β-TCP)/chitosan (CS)/polycaprolactone (PCL) cage+ ADSCs], TE cage group (β-TCP/CS/PCL cage+ ADSCs), non-modified β-TCP/CS/PCL cage, hydroxyapatite (HA) cage, titanium and bone allograft cage. Three-dimensional CT was used to observe morphology changing, bone mineralization ratio, new bone mension and osteoblatic quantity, interleukin-6 (IL-6), metalloprotease-1 (MMP-1), tumor necrosis factor-α (TNF-α) and tartrate resistant acid phosphatase (TRAP), and osteoprotein (OPG) gene expression were detected at 2, 4, 8, 12, 16, 20, 24, 28 and 36 weeks. Biomechanical property was also detected at different interval.
Results
Compared with other groups, implant subsidence and migration were not found in surface-modified TE cage group. Meanwhile, new bone prolifreated remarkably with satisfactory data of bone mineralization ratio, and new bone mension or osteoblatic quantity [(63.9±1.3)%, (76.54±1.8)%, (73.8±2.3) cells]. IL-6, MMP-1, TNF-α and TRAP gene downregulated (0.09±0.01, 0.13±0.02, 0.08±0.01). Conversely, Runx2 and OPG gene upregulated (0.73±0.13, 0.57±0.16). In addition, data of bone strength and rang of motion in surface-modified TE cage group were significantly higher than those of other groups [(4.38±0.25) Nm/degree, (6.55±0.19)°].
Conclusion
Invertebral osteolysis can be effectively controlled by surface-modified TE approach, and this provides a potential therapy for implant aseptic loosening.
Key words:
Osteolysis; Cage; Surface modification; Tissue engineering; Interface
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