用阳离子交换多模态吸附剂分离重组人促红细胞生成素

IF 0.9 Q4 CHEMISTRY, MULTIDISCIPLINARY Acta Chimica Slovaca Pub Date : 2019-04-01 DOI:10.2478/acs-2019-0015
Marta Ostrihoňová, Jana Adamíková, T. Molnár, M. Antošová, M. Polakovič
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引用次数: 0

摘要

摘要:本工作涉及从浓缩培养后上清中的混合蛋白中捕获人重组红细胞生成素(rhEPO)。选择阳离子交换多模态吸附剂Capto MMC ImpRes作为潜在的色谱分离材料。通过间歇吸附实验研究了其平衡性能。考察了pH在5.5 ~ 7.5范围内、NaCl浓度在0 ~ 300 mM范围内对rhEPO和污染物蛋白吸附的影响。在这些平衡实验中找到的最佳条件适用于rhEPO在色谱柱上的吸附。在不同的洗脱条件下,对rhEPO的收率和选择性进行了优化。
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Recombinant human erythropoietin separation using a cation-exchange multimodal adsorbent
Abstract This work deals with the capture of human recombinant erythropoietin (rhEPO) from a mixture of proteins in a concentrated postcultivation supernatant. Cation-exchange multimodal adsorbent Capto MMC ImpRes was selected as potential chromatographic separation material. Its equilibrium properties were investigated in batch adsorption experiments. The effect of pH in the range of 5.5—7.5 and NaCl concentration in the range of 0—300 mM on the adsorption of rhEPO and contaminant proteins was examined. Optimal conditions found in these equilibrium experiments were applied to rhEPO adsorption in a chromatographic column. Several experiments were carried out at different elution conditions to optimize the rhEPO yield and selectivity.
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Acta Chimica Slovaca
Acta Chimica Slovaca CHEMISTRY, MULTIDISCIPLINARY-
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11
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