威卡病毒感染克隆及玉米绿矮病毒绿色荧光蛋白的维管表达

IF 2 Q4 VIROLOGY Frontiers in virology Pub Date : 2023-06-29 DOI:10.3389/fviro.2023.1216285
L. Stewart, K. Willie, Wenshuang Xie, J. Todd, Hong Hanh Tran
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引用次数: 0

摘要

威卡病毒属植物病毒是一种正感单链RNA病毒,包括玉米的重要病原体(玉米绿矮病毒;MCDV)和水稻(水稻结核球形病毒;RTSV)。由于实验挑战和缺乏低滴度、韧皮部受限和专性媒介传播的waikvirus的传染性克隆,waikvirus的分子生物学的许多方面仍未被探索。本文首次获得了MCDV- s和MCDV- m1两个MCDV株的威卡病毒感染克隆,并通过维管穿刺接种将这些克隆在玉米中无虫启动。我们进一步用绿色荧光蛋白(GFP)编码序列取代病毒p27编码序列,开发了一个绿色荧光蛋白(GFP)标记的MCDV克隆。GFP标记的病毒在植物中系统移动,引起与野生型病毒相似的症状感染,具有血管表达GFP。怀卡病毒感染性克隆的开发是这类具有重要农业意义的病毒的一个重大进展。
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First waikavirus infectious clones and vascular expression of green fluorescent protein from maize chlorotic dwarf virus
Plant viruses classified in the genus Waikavirus, family Secoviridae, are positive sense single-stranded RNA viruses that include important pathogens of maize (maize chlorotic dwarf virus; MCDV) and rice (rice tungro spherical virus; RTSV). Many aspects of the molecular biology of waikaviruses remain unexplored because of experimental challenges and lack of infectious clones for low titer, phloem-limited, and obligately vector-transmitted waikaviruses. Here we report the first development of waikavirus infectious clones for two MCDV strains, MCDV-S and MCDV-M1, and insect-free launching of infections from these clones in maize by vascular puncture inoculation. We further developed a green fluorescent protein (GFP)-tagged MCDV clone by replacing the viral p27-encoding sequence with GFP-encoding sequence. GFP-tagged virus moved systemically in plants and caused symptomatic infection similar to wild type virus, with vascular expression of GFP. Development of waikavirus infectious clones is a major advance for this group of agriculturally significant viruses.
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