THE EFFECTS OF COMMON HOUSE FLY (MUSCA DOMESTICA) LARVAE-DERIVED SUBSTANCES ON WOUND HEALING IN MOUSE MODEL

Background: Maggot debridement therapy is a therapeutic wound myiasis that depends mainly on L. sericata larva. Owing to its availability and cheap breeding, M. domestica was suggested as an alternative to Lucilia . Objective: The present study was designed to assess the role of M. domestica larvae-derived substances on wound healing in immunocompetent and immunosuppressed mice. Material and Methods: Chitosan and gut extracts, obtained from M. domestica larvae, were applied daily on skin wounds of Swiss albino mice. Mice were divided into two groups, control non-treated mice (I), and experimental treated mice (II). Each group included immunocompetent (a), and immunosuppressed (b) mice. The experimental subgroups were treated with either chitosan (1) or gut extracts (2). Wounds were assessed by macroscopic evaluation, wound contracture, histopathological, immunohistochemical and bacteriological parameters. Results: In control non treated mice hair growth was evident with normal underlying skin by the end of the experiment (4 weeks) except in immunosuppressed (Ib) subgroup. Significant reduction in wound size was detected on the 7 th day post wounding (PW) in the immunocompetent-chitosan-treated subgroup