利用免疫分析法和血清及初乳折光计测定血液中IgG水平监测新生牛犊被动免疫转移

Osman A. Hameed, Hussam Mustafa, Abdul Fatah M. Ahmed, M. Taha
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引用次数: 0

摘要

IgG检查小牛试验已在本研究中使用,以确定是否失败的被动转移发生在新生牛犊通过测量血液中的IgG水平。所有犊牛出生后48小时、7天和14天的IgG水平均为1000 mg/dl。这表明它们具有良好的被动免疫转移。此外,本研究还采用白度折光仪检测犊牛初乳质量。初乳是高质量的,因为它含有50毫克/毫升的IgG。这种Brix折光计可以在农场水平上用于估计初乳IgG含量和监测初乳喂养方法。含有高质量IgG的初乳可以为犊牛提供足够的IgG,从而实现免疫的成功被动转移。Brix和Obione折射仪提供了一种简单、快速的方法来估计犊牛血清中IgG的浓度,因此被认为是确定被动转移失败的最常用方法。
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Monitoring Passive Transfer of Immunity in Neonatal Calves by Measuring Levels of IgG in Blood Using Immunoassay Method and Refractometer Measures of Serum and Colostrum
IgG Check calf test has been used in this study to identify if a failure of passive transfer occurs in neonatal calves by measuring the level of IgG in blood. An adequate level of IgG measured in all calves at 48 hours, 7 days and 14 days after birth showed level of IgG 1000 mg/dl. This level indicated that they have good passive transfer of immunity. Also, in this study colostrum quality fed to calves was detected using Brix Refractometer. The colostrum is of high quality as it contains 50 mg/ml of IgG. This Brix refractometer can be used on the farm level to estimate colostrum IgG content and monitoring colostrum feeding practices. Colostrum with high quality IgG could provide calves with enough IgG to attain successful passive transfer of immunity. Brix and Obione refractometers provide simple, rapid method for estimating IgG concentration on calf serum, thus considered to be the most common method for determining passive transfer failure.
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