收集马和马鬃提取物GC-MS光谱中未知峰信息的试验

M. Tsikolia, T. Opatz, U. Kauhl, N. Tabanca, B. Demirci, S. TenBroeck, K. Linthicum, U. Bernier
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引用次数: 2

摘要

通过对马、小马毛发和皮肤中挥发性化合物的分析,确定了食血双翅目昆虫用于定位寄主的生物活性分子。本研究采用有机溶剂(戊烷或己烷)提取马毛样品,并采用封闭电子电离(CEI)气相色谱-质谱分析(GC-MS)进行分析。通过GC-MS分析,我们观察到一个复合峰,该峰无法通过与质谱库中的质谱进行比较来识别。这种未知的化合物通常是色谱中最大的峰,其保留指数和质谱碎片类似于壬醛。然而,在破碎化模式上存在一些差异。当与非肛门参考标准比较时,很明显这是一种不同的化合物。马鬃加氢蒸馏法是另一种获得高浓度未知成分的提取方法。用气相色谱-火焰离子化检测器(FID)和气相色谱-质谱联用分析证实了相同的未知峰。用各种质谱工具进行进一步的实验和分析,得出了可能的分子离子,其质量电荷比(m/z)为142,与未知成分相对应。
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Trials for Gathering Information on an Unknown Peak in the GC-MS Spectra of Horse and Pony Hair Extracts
The volatile compounds from horse and pony hairs and skin were analyzed to determine bioactive molecules that are kairomones used for host location by blood-feeding diptera. In this study, horse and pony hair samples were extracted with organic solvents (pentane or hexane) and analyzed by gas chromatography-mass spectrometry analysis (GC-MS) with closed electron ionization (CEI). Using the GC-MS analysis, we observed a compound peak, which could not be identified by comparison to mass spectra in the mass spectral library. This unknown compound was often the largest peak in the chromatogram and had a retention index and mass spectral fragmentation similar to nonanal. However, there were several differences in the fragmentation pattern. When compared to a nonanal reference standard, it was evident that this was a different compound. Hydrodistillation of pony hair was another extraction method to obtain the unknown component in higher concentrations. Analysis of this extract with GC-flame ionization detector (FID) with GC-MS confirmed the same unknown peak. Further experiments and analysis with the various mass spectroscopy tools gave the possible molecular ion with mass-to-charge ratio (m/z) 142, corresponding to the unknown component.
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