甘露聚糖结合凝集素2基因多态性变异与慢性牙周炎易感性的遗传关联及上位互作分析

IF 0.8 Q4 GENETICS & HEREDITY Meta Gene Pub Date : 2021-12-01 DOI:10.1016/j.mgene.2021.100963
Bushra Butul , Nusrath Fathima , Sandeep Kumar Vishwakarma , Aleem Ahmed Khan
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引用次数: 0

摘要

目的探讨印度慢性牙周炎(CP)患者CD14与甘露聚糖结合凝集素2 (MBL2)基因多态性变异的相关性及上位性相互作用。我们共招募了242名受试者(121名患者和121名对照受试者),年龄在35至60岁之间,无论性别和CD14 (- 159C >T, NC_000005.10: g。2569190: C比;T)和MBL2(密码子52,C >T, NM_000242: c。52 c比;T)外周血样本中的多态变异。采用多因子降维(MDR)方法进行了互作分析,并使用Genebee在线RNA工具预测了MBL2基因的核糖核酸(RNA)结构。结果慢性牙周炎患者CD14和MBL2基因多态性的“CT”杂合子和变异等位基因“T”的频率显著增加。MDR分析显示,CP的风险增加了大约两倍。计算机分析显示,在CD14 (-159C >T)多态性。MBL2(密码子52,C >T)多态性表现出具有近似相似自由能的结构变化。结论基因型“CT”杂合子和变异的“T”等位基因对CP患者CD14和MBL2基因多态性均有显性作用,“T”等位基因的存在导致CD14 (-159C >T)和RNA分子排列的改变可能进一步影响CD14和MBL2基因的表达,从而增加对CP发病机制的易感性。
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Genetic association and epistatic interaction analysis of cluster of differentiation 14 and mannan-binding lectin 2 gene polymorphic variants in susceptibility to chronic periodontitis

Objective

To study the association and epistatic interactions of cluster of differentiation 14 (CD14) and mannan-binding lectin 2 (MBL2) gene polymorphic variants in Indian patients with chronic periodontitis (CP).

Design

We enrolled a total of 242 individuals (121 patients and 121 control subjects), age 35 to 60 years both irrespective of gender and identified CD14 (−159C > T, NC_000005.10:g.2569190:C > T) and MBL2 (codon 52, C > T, NM_000242:c.52C > T) polymorphic variants in peripheral blood samples. We also performed epistatic interaction analysis using multifactor dimensionality reduction (MDR) approach and predicted ribonucleic acid (RNA) structure of MBL2 gene using Genebee online RNA tool.

Results

Significantly increased frequency of ‘CT' heterozygote and variant allele ‘T' in chronic periodontitis patients was observed for both CD14 and MBL2 gene polymorphisms. MDR analysis showed approximately two-fold increased risk of CP. In silico analysis showed lack of transcription factor ETF (TEA domain family member 2) binding-site in presence of ‘T' allele ‘in CD14 (-159C > T) polymorphism. The secondary RNA structure prediction of MBL2 (codon 52, C > T) polymorphism showed structural variations having approximately similar free energies.

Conclusions

A dominant effect of genotype ‘CT' heterozygote and variant ‘T' allele observed for both CD14 and MBL2 gene polymorphisms in patients with CP. The presence of ‘T' allele also results in lack of transcription factor binding site at CD14 (-159C > T) and changes in arrangements of RNA molecules that may further affect expression of CD14 and MBL2 genes leading to increased susceptibility to CP pathogenesis.

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来源期刊
Meta Gene
Meta Gene Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
1.10
自引率
0.00%
发文量
20
期刊介绍: Meta Gene publishes meta-analysis, polymorphism and population study papers that are relevant to both human and non-human species. Examples include but are not limited to: (Relevant to human specimens): 1Meta-Analysis Papers - statistical reviews of the published literature of human genetic variation (typically linked to medical conditionals and/or congenital diseases) 2Genome Wide Association Studies (GWAS) - examination of large patient cohorts to identify common genetic factors that influence health and disease 3Human Genetics Papers - original studies describing new data on genetic variation in smaller patient populations 4Genetic Case Reports - short communications describing novel and in formative genetic mutations or chromosomal aberrations (e.g., probands) in very small demographic groups (e.g., family or unique ethnic group). (Relevant to non-human specimens): 1Small Genome Papers - Analysis of genetic variation in organelle genomes (e.g., mitochondrial DNA) 2Microbiota Papers - Analysis of microbiological variation through analysis of DNA sequencing in different biological environments 3Ecological Diversity Papers - Geographical distribution of genetic diversity of zoological or botanical species.
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