采用本地谱库和“自制”内标进行HPLC-DAD毒理学筛选

K. Sobhi, Souhila Mélaïne, Abderrahmane Abdaoui, M. Mammar
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引用次数: 0

摘要

采用液-液萃取技术和HPLC-DAD法,采用C18柱,5μm(150x4.6mm i.d.)进行毒理学药物筛选。采用国产“N-乙酰帕罗西汀”作为内标。建立了一个由130多种药物及其一些代谢产物组成的局部紫外光谱库。该图书馆涵盖了涉及中毒病例的主要治疗类别。开发的程序成功应用于不同的生物样本(血清、尿液、胃液和死后全血),可以快速、同时检测和鉴定血清中的大量物质及其半定量。
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Toxicological screening method by HPLC-DAD with local spectral library and "home-made" internal standard
A liquid-liquid extraction technique and HPLC-DAD method using a C18 column, 5μm (150x4.6mm i.d.) were developed for a toxicological drug screening. A home-made “N-acetylparoxetine” is used as internal standard. A local UV spectra library of over 130 drugs and some of their metabolites was created. This library covers the main therapeutic classes involved in intoxication cases. The developed procedure is successfully applied to different biological samples (serum, urine, gastric fluid and post mortem whole blood) and allows rapid and simultaneous detection and identification of a large number of substances and their semi-quantification in serum.
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