Zeinab Naeini, Faezeh Abaj, Z. Esmaeily, E. Alvandi, Masoumeh Rafiee, F. Koohdani
{"title":"研究ApoB-EcoR1多态性的营养遗传学方法——饮食酸负荷相互作用对2型糖尿病成人血脂异常和人体测量相关结果的影响","authors":"Zeinab Naeini, Faezeh Abaj, Z. Esmaeily, E. Alvandi, Masoumeh Rafiee, F. Koohdani","doi":"10.1159/000528656","DOIUrl":null,"url":null,"abstract":"Introduction: Despite multiple studies which have considered the role of dietary acid load (DAL) or Apolipoprotein B (ApoB) EcoR1 polymorphism in diabetes, none have assessed their interplay effect on metabolic markers. Therefore, this study aimed to determine the interaction of EcoR1 and DAL on metabolic markers among adults with Type 2 diabetes mellitus (T2DM).\nMethods: 492 randomly selected individuals with T2DM were recruited for this cross-sectional study. Dietary intake was evaluated by a validated food frequency questionnaire. DAL was assessed as net-endogenous acid production (NEAP) and potential renal acid load (PRAL). Real-time-PCR was used to genotype EcoR1. Metabolic markers were also assessed. The interaction effect of the polymorphism and DAL indexes was analyzed by analysis of covariance (ANCOVA). \nResult: The frequency of EcoR1 genotypes was not different between dyslipidemic and normolipidemic participants (P>0.05). Among participants with dyslipidemia, those with the GG genotype and who consumed a higher level of NEAP had higher body mass index (BMI) (p=0.03) and waist circumference (WC; p =0.02). Moreover, triglyceride (TG) concentration (P=0.007), the LDL/HDL ratio (P=0.03) and the TG/HDL (P=0.03) ratio were significantly higher in A allele carriers with higher than the median intake of NEAP, in comparison with GG homozygotes. Finally, GA/AA carriers who had a higher intake of PRAL had a higher TG concentration (P=0.006) and TG/HDL ratio (P=0.01) compared to lower median intake in the dyslipidemia group. \nDiscussion/Conclusion: In the dyslipidemic group, there was a higher TG concentration among individuals with the GA/AA genotype and a higher intake of NEAP/ PRAL. Also, in this group, a higher intake of NEAP may be considered as a risk factor for increased levels of BMI and WC among participants with the GG genotype.\n\n\n","PeriodicalId":18030,"journal":{"name":"Lifestyle Genomics","volume":"1 1","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2022-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"A Nutrigenetic Approach to Investigate ApoB EcoR1 Polymorphism–Dietary Acid Load Interactions on Lipid and Anthropometric-Related Outcomes in Adults with Dyslipidemic Type 2 Diabetes\",\"authors\":\"Zeinab Naeini, Faezeh Abaj, Z. Esmaeily, E. Alvandi, Masoumeh Rafiee, F. Koohdani\",\"doi\":\"10.1159/000528656\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Introduction: Despite multiple studies which have considered the role of dietary acid load (DAL) or Apolipoprotein B (ApoB) EcoR1 polymorphism in diabetes, none have assessed their interplay effect on metabolic markers. Therefore, this study aimed to determine the interaction of EcoR1 and DAL on metabolic markers among adults with Type 2 diabetes mellitus (T2DM).\\nMethods: 492 randomly selected individuals with T2DM were recruited for this cross-sectional study. Dietary intake was evaluated by a validated food frequency questionnaire. DAL was assessed as net-endogenous acid production (NEAP) and potential renal acid load (PRAL). Real-time-PCR was used to genotype EcoR1. Metabolic markers were also assessed. The interaction effect of the polymorphism and DAL indexes was analyzed by analysis of covariance (ANCOVA). \\nResult: The frequency of EcoR1 genotypes was not different between dyslipidemic and normolipidemic participants (P>0.05). Among participants with dyslipidemia, those with the GG genotype and who consumed a higher level of NEAP had higher body mass index (BMI) (p=0.03) and waist circumference (WC; p =0.02). Moreover, triglyceride (TG) concentration (P=0.007), the LDL/HDL ratio (P=0.03) and the TG/HDL (P=0.03) ratio were significantly higher in A allele carriers with higher than the median intake of NEAP, in comparison with GG homozygotes. Finally, GA/AA carriers who had a higher intake of PRAL had a higher TG concentration (P=0.006) and TG/HDL ratio (P=0.01) compared to lower median intake in the dyslipidemia group. \\nDiscussion/Conclusion: In the dyslipidemic group, there was a higher TG concentration among individuals with the GA/AA genotype and a higher intake of NEAP/ PRAL. Also, in this group, a higher intake of NEAP may be considered as a risk factor for increased levels of BMI and WC among participants with the GG genotype.\\n\\n\\n\",\"PeriodicalId\":18030,\"journal\":{\"name\":\"Lifestyle Genomics\",\"volume\":\"1 1\",\"pages\":\"\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2022-12-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Lifestyle Genomics\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1159/000528656\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Lifestyle Genomics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1159/000528656","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
A Nutrigenetic Approach to Investigate ApoB EcoR1 Polymorphism–Dietary Acid Load Interactions on Lipid and Anthropometric-Related Outcomes in Adults with Dyslipidemic Type 2 Diabetes
Introduction: Despite multiple studies which have considered the role of dietary acid load (DAL) or Apolipoprotein B (ApoB) EcoR1 polymorphism in diabetes, none have assessed their interplay effect on metabolic markers. Therefore, this study aimed to determine the interaction of EcoR1 and DAL on metabolic markers among adults with Type 2 diabetes mellitus (T2DM).
Methods: 492 randomly selected individuals with T2DM were recruited for this cross-sectional study. Dietary intake was evaluated by a validated food frequency questionnaire. DAL was assessed as net-endogenous acid production (NEAP) and potential renal acid load (PRAL). Real-time-PCR was used to genotype EcoR1. Metabolic markers were also assessed. The interaction effect of the polymorphism and DAL indexes was analyzed by analysis of covariance (ANCOVA).
Result: The frequency of EcoR1 genotypes was not different between dyslipidemic and normolipidemic participants (P>0.05). Among participants with dyslipidemia, those with the GG genotype and who consumed a higher level of NEAP had higher body mass index (BMI) (p=0.03) and waist circumference (WC; p =0.02). Moreover, triglyceride (TG) concentration (P=0.007), the LDL/HDL ratio (P=0.03) and the TG/HDL (P=0.03) ratio were significantly higher in A allele carriers with higher than the median intake of NEAP, in comparison with GG homozygotes. Finally, GA/AA carriers who had a higher intake of PRAL had a higher TG concentration (P=0.006) and TG/HDL ratio (P=0.01) compared to lower median intake in the dyslipidemia group.
Discussion/Conclusion: In the dyslipidemic group, there was a higher TG concentration among individuals with the GA/AA genotype and a higher intake of NEAP/ PRAL. Also, in this group, a higher intake of NEAP may be considered as a risk factor for increased levels of BMI and WC among participants with the GG genotype.
期刊介绍:
Lifestyle Genomics aims to provide a forum for highlighting new advances in the broad area of lifestyle-gene interactions and their influence on health and disease. The journal welcomes novel contributions that investigate how genetics may influence a person’s response to lifestyle factors, such as diet and nutrition, natural health products, physical activity, and sleep, amongst others. Additionally, contributions examining how lifestyle factors influence the expression/abundance of genes, proteins and metabolites in cell and animal models as well as in humans are also of interest. The journal will publish high-quality original research papers, brief research communications, reviews outlining timely advances in the field, and brief research methods pertaining to lifestyle genomics. It will also include a unique section under the heading “Market Place” presenting articles of companies active in the area of lifestyle genomics. Research articles will undergo rigorous scientific as well as statistical/bioinformatic review to ensure excellence.