光电二极管阵列快速反相液相色谱法测定片剂中氯噻酮、琥珀酸美托洛尔和替米沙坦的含量

IF 1.8 Q3 CHEMISTRY, ANALYTICAL Journal of Chemical Metrology Pub Date : 2021-08-24 DOI:10.25135/jcm.6012106.2098
Avani Chokshi, R. Prajapati, Pritesh Desai, Mehul D. Patel
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引用次数: 1

摘要

建立了一种快速简便的反相高效液相色谱法(RP-HPLC),用于同时定量测定原料药和片剂中氯噻酮、琥珀酸美托洛尔和替米沙坦的含量。采用Inertsil Octadecyl-Silica (ODSC18)柱(150mm×4.6mm, 5μm)固定相进行色谱分离。采用磷酸缓冲液(50 mM, pH 2.5)与乙腈的流动相混合物,以40:60 v/v和0.7 mL/min流速进行分离和洗脱。氯噻酮、琥珀酸美托洛尔和替米沙坦洗脱时间分别为2.96±0.5 min、4.31±0.5 min和5.94±0.5 min。利用光电二极管阵列(PDA)探测器在225 nm波长处记录响应。所选方法在3.21 ~ 18.72 μg/mL、6.25 ~ 37.50 μg/mL和10 ~ 60 μg/mL范围内呈线性关系,氯噻酮、琥珀酸美托洛尔和替米沙坦的回收率分别为99.15 ~ 99.93%、99.15 ~ 99.42%和100.03 ~ 100.08%。结果表明,该方法具有灵敏度高、选择性好、线性好、重复性好等特点,适用于复方制剂的常规分析。
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Development of a quick reverse phase liquid chromatographic method with photodiode-array detection for quantitative determination of chlorthalidone, metoprolol succinate and telmisartan in tablet formulation
A quick and simple reversed-phase High-Performance Liquid Chromatographic method (RP-HPLC) with isocratic elution has been developed and validated for the concurrent quantitative determination of chlorthalidone, metoprolol succinate and telmisartan in the bulk mixture and tablet dosage form. The chromatographic separation was performed using Inertsil Octadecyl-Silica (ODSC18) Column (150mm×4.6mm, 5μm) stationary phase. The separation and elution was carried out using a mobile phase mixture of phosphate buffer (50 mM, pH 2.5) and acetonitrile at a ratio of 40:60 v/v and 0.7 mL/min flow rate. Chlorthalidone, metoprolol succinate and telmisartan were eluted at 2.96±0.5 min, 4.31±0.5 min and 5.94±0.5 min respectively. The response was recorded using a photodiode array (PDA) detector at 225 nm wavelength. The selected method was linear in the range of 3.21-18.72 μg/mL, 6.25-37.50 μg/mL and 10-60 μg/mL while percentage recovery was in the range from 99.15 to 99.93%, 99.15 to 99.42% and 100.03 to 100.08% for chlorthalidone, metoprolol succinate and telmisartan respectively. The method was found to be sensitive, selective, linear and reproducible and the results obtained suggest the suitability of the developed method for routine analysis of the formulations containing the combination of these drugs.
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来源期刊
Journal of Chemical Metrology
Journal of Chemical Metrology CHEMISTRY, ANALYTICAL-
CiteScore
2.30
自引率
15.40%
发文量
7
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