{"title":"从大豆酱中分离的曲霉产黄曲霉毒素的方法验证","authors":"S. Yoo, W. Jeong, S. Yeo, So-Young Kim","doi":"10.11002/kjfp.2023.30.1.28","DOIUrl":null,"url":null,"abstract":"\n \n Non-aflatoxigenic Aspergillus oryzae and aflatoxigenic\n A. flavus cannot be clearly identified by partial\n sequencing of the internal transcribed spacer (ITS) and 18S ribosomal\n ribonucleic acid (18S rRNA) regions. This study aimed to compare the accuracy\n among three aflatoxin detection methods using ultra-performance liquid\n chromatography (UPLC), high-performance liquid chromatography (HPLC), and an\n enzyme-linked immunosorbent assay (ELISA) kit and to select the\n non-aflatoxigenic Aspergillus sp. isolated from soybean paste.\n All analytical methods were suitable according to the international standards of\n Codex Alimentarius FAO-WHO (CODEX) or the Ministry of Food and Drug Safety\n (MFDS). UPLC exhibited the best of limit of detection (LOD) and limit of\n quantification (LOQ). Based on UPLC, HPLC, and the ELISA kit assay, the P5 and\n P7 strains isolated from soybean paste had 1,663.49, 1,468.12, and ⟩20\n μg/kg and 1,470.08, 1,056.73, and ⟩20\n μg/kg, respectively, detected and re-identified as\n A. flavus. In contrast, the P3 and P4 strains (A.\n oryzae), which were detected below the MFDS standards in all\n assays, were confirmed as non-aflatoxigenic fungi. Among the methods evaluated\n for quantitative analysis of aflatoxin, UPLC and HPLC are superior in terms of\n accuracy, and the ELISA kit rapidly detects low concentrations of aflatoxin.\n Furthermore, this study demonstrates that any Aspergillus sp.\n isolated for use as a fermentation starter should be analyzed for potential\n aflatoxin production using UPLC and HPLC for accurate quantitative analysis or\n ELISA for the rapid detection of low-level concentrations of aflatoxin.\n","PeriodicalId":17875,"journal":{"name":"Korean Journal of Food Preservation","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Method validation for quantitative analyzing aflatoxin productivity\\n in Aspergillus sp. isolated from soybean paste\",\"authors\":\"S. Yoo, W. Jeong, S. Yeo, So-Young Kim\",\"doi\":\"10.11002/kjfp.2023.30.1.28\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"\\n \\n Non-aflatoxigenic Aspergillus oryzae and aflatoxigenic\\n A. flavus cannot be clearly identified by partial\\n sequencing of the internal transcribed spacer (ITS) and 18S ribosomal\\n ribonucleic acid (18S rRNA) regions. This study aimed to compare the accuracy\\n among three aflatoxin detection methods using ultra-performance liquid\\n chromatography (UPLC), high-performance liquid chromatography (HPLC), and an\\n enzyme-linked immunosorbent assay (ELISA) kit and to select the\\n non-aflatoxigenic Aspergillus sp. isolated from soybean paste.\\n All analytical methods were suitable according to the international standards of\\n Codex Alimentarius FAO-WHO (CODEX) or the Ministry of Food and Drug Safety\\n (MFDS). UPLC exhibited the best of limit of detection (LOD) and limit of\\n quantification (LOQ). Based on UPLC, HPLC, and the ELISA kit assay, the P5 and\\n P7 strains isolated from soybean paste had 1,663.49, 1,468.12, and ⟩20\\n μg/kg and 1,470.08, 1,056.73, and ⟩20\\n μg/kg, respectively, detected and re-identified as\\n A. flavus. In contrast, the P3 and P4 strains (A.\\n oryzae), which were detected below the MFDS standards in all\\n assays, were confirmed as non-aflatoxigenic fungi. Among the methods evaluated\\n for quantitative analysis of aflatoxin, UPLC and HPLC are superior in terms of\\n accuracy, and the ELISA kit rapidly detects low concentrations of aflatoxin.\\n Furthermore, this study demonstrates that any Aspergillus sp.\\n isolated for use as a fermentation starter should be analyzed for potential\\n aflatoxin production using UPLC and HPLC for accurate quantitative analysis or\\n ELISA for the rapid detection of low-level concentrations of aflatoxin.\\n\",\"PeriodicalId\":17875,\"journal\":{\"name\":\"Korean Journal of Food Preservation\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Korean Journal of Food Preservation\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.11002/kjfp.2023.30.1.28\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Korean Journal of Food Preservation","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.11002/kjfp.2023.30.1.28","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Method validation for quantitative analyzing aflatoxin productivity
in Aspergillus sp. isolated from soybean paste
Non-aflatoxigenic Aspergillus oryzae and aflatoxigenic
A. flavus cannot be clearly identified by partial
sequencing of the internal transcribed spacer (ITS) and 18S ribosomal
ribonucleic acid (18S rRNA) regions. This study aimed to compare the accuracy
among three aflatoxin detection methods using ultra-performance liquid
chromatography (UPLC), high-performance liquid chromatography (HPLC), and an
enzyme-linked immunosorbent assay (ELISA) kit and to select the
non-aflatoxigenic Aspergillus sp. isolated from soybean paste.
All analytical methods were suitable according to the international standards of
Codex Alimentarius FAO-WHO (CODEX) or the Ministry of Food and Drug Safety
(MFDS). UPLC exhibited the best of limit of detection (LOD) and limit of
quantification (LOQ). Based on UPLC, HPLC, and the ELISA kit assay, the P5 and
P7 strains isolated from soybean paste had 1,663.49, 1,468.12, and ⟩20
μg/kg and 1,470.08, 1,056.73, and ⟩20
μg/kg, respectively, detected and re-identified as
A. flavus. In contrast, the P3 and P4 strains (A.
oryzae), which were detected below the MFDS standards in all
assays, were confirmed as non-aflatoxigenic fungi. Among the methods evaluated
for quantitative analysis of aflatoxin, UPLC and HPLC are superior in terms of
accuracy, and the ELISA kit rapidly detects low concentrations of aflatoxin.
Furthermore, this study demonstrates that any Aspergillus sp.
isolated for use as a fermentation starter should be analyzed for potential
aflatoxin production using UPLC and HPLC for accurate quantitative analysis or
ELISA for the rapid detection of low-level concentrations of aflatoxin.
期刊介绍:
This journal aims to promote and encourage the advancement of quantitative improvement for the storage, processing and distribution of food and its related disciplines, theory and research on its application. Topics covered include: Food Preservation and Packaging Food and Food Material distribution Fresh-cut Food Manufacturing Food processing Technology Food Functional Properties Food Quality / Safety.